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Methods Serum TGFβ1 and TIMP-1 were detected by en- zyme-linked immunosorbent assay and serum HA,LN,PⅢNP were detected by radioimmunoassay in 40 CHB patients and 12 healthy persons. Liver biopsies were performed on the 40 patients.
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方法采用酶联免疫吸附法 (ELISA)检测40例慢性乙型肝炎患者和12名健康体检者血清TGFβ1、TIMP-1水平,RIA检测血清HA,LN、Ⅲ型前胶原氨基端肽(P．Ⅲ NP)水平,40例患者全部进行肝活组织检查,分析TGFβ1、TIMP-1与肝组织纤维化程度分期和炎症活动度分级的关系以及五项指标之间的相关性。 |
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Methods Serum TNF-α and IL-6 were determined by enzyme-linked immunosorbent assay in normal subjects(control group),UA patients treated with and without rosiglitazone.
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方法采用酶联免疫吸附法(ELISA法)测定正常对照组、UA患者罗格列酮干预组和未干预组血清中IL-6、TNF-α水平的变化。 |
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Methods Serum levels of VEGF, VEGF C and uPAR were measured with quantitative enzyme-linked immunosorbent assay in 91 patients with OSCC and in 50 healthy individuals as control group.
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方法用酶联免疫吸附分析法检测91例OSCC患者和50例健康人血清中VEGF、VEGF C和uPAR的水平。 |
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Methods Serum sFas and sFasL were assayed by Enzyme-linked immunosorbent assays(ELISA) in 50 patients with gastric cancer, 36 cases with benign gastric diseases and 32 cases of normal subjects.
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方法:采用酶联免疫吸附法(ELISA)检测50例胃癌患者,36例胃良性疾病和32例正常健康者血清中sFas、sFasL的水平,并比较胃癌组中不同TNM分期的变化。 |
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Methods Test the activity of CD69 and level of IL-2 by cell flow cytometer after colon cancer operation in 33 cases.
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方法应用流式细胞仪检测33例结肠癌患者手术前后外周血中T淋巴细胞CD69的表达百分率及IL-2的表达水平,并与对照组进行比较分析。 |
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Methods The HBV DNA in HepG 2.2.15 cell line supernatant with/without IFNα-2b were monitored by fluorescence real-time quantitive PCR.
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方法 用定量聚合酶链反应(PCR)检测IFNα2b处理前、后的HepG2 2 15细胞上清的乙型肝炎病毒脱氧核糖核酸(HBVDNA)水平。 |
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Methods The MK levels in serum were determined with enzyme-linked immunosorbent assays for 20 healthy women,20 patients with breast benign diseases and 64 patients with breast cancer.
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方法采用酶联免疫吸附法对20例正常女性,20例女性乳腺良性疾病和64例乳腺癌患者血清MK进行检测。 |
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Methods The TK6 cell was cultured and its genomic DNA was extracted and prepared to the single-stranded probes of the exon 2 of k-ras gene.
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方法培养TK6细胞,抽提基因组DNA,制备k-ras基因外显子2的单链探针。 |
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Methods The allergen of inhalation and edibility in 311 cases with allergic dermatitis and 30 healthy persons were detected by enzyme-linked immunosorbent assay (ELISA).
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方法采用酶联免疫吸附试验(ELISA)对311例过敏性皮肤病患者和30名健康对照者血清中吸入性及食入性过敏原进行检测。 |
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Methods The effects of dopamine (DA) and glutathione (GSH) on apoptotic PC12 cell were examined by fluorescence microscopy using the DNA end- labeling method.
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方法用脱氧核糖核酸（DNA）标记法，借助荧光显微镜观察多巴胺（DA）和还原型谷胱甘肽（GSH）对PC12细胞凋亡的影响。 |
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Methods The expression levels of the P53 protein and Ki-67 were examined by immunohistochemistry(S-P method). To analysis the relationship between levels of P53 protein and Ki-67 and differentiation,clinical stage and metastasis of esophageal carcinoma.
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方法应用免疫化学S-P法对50例食管鳞状细胞癌中P53蛋白和Ki-67的表达水平进行研究,分析其与食管癌组织分化程度、临床分期及转移的关系。 |
