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| Methods GHcDNA was cleaved by two kinds of restriction enzyme from pUC9-GHcDNA and inserted into eukaryotic expression vector pcDNA ,froming recombinant pcDNA -GH plastid. Its sequence was identified by cleaving of enzyme and sequencing. |
中文意思：
方法 用双酶切从 pUC9-GHcDNA中克隆出生长激素基因 ,然后定向插入真核细胞表达载体pcDNA 中 ,行酶切和测序以鉴定。 |
以下为相关句子列表(点击查看，可以看对应的解释）：
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Methods Ex vivo incision experiment was performed on 8 lumbar discs of pigs using rotating pliers.
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方法 体外试验 :用 只猪 8个腰椎间盘进行器械的切割实验。 |
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Methods Forty children scheduled for squint correction surgery were randomly divided into the propofol group (group P,n=0) and the ketamine group (group K,n=0).
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方法 :将 0例患儿随机分成异丙酚组(P组 ,n = 0 )和氯胺酮组 (K组 ,n = 0 )。 |
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Methods From 97～99, cases of median nerve injury on high level were treated to reconstruct the opponent pollex by replacing flexor pollicis longus with extensor carpiradialis longus;
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方法 对 97年 月至 99年 0月间采用桡侧腕长伸肌代拇长屈肌 ; |
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Methods From 990 to 00 , 9 patients with pectus carinatum were treated, whose ages ranged from years and months to years.
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方法 990年～ 0 0 年收治鸡胸患者9例,年龄 岁个月～岁。 |
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Methods From September, 00 to September, 00 , the labia minora flap vaginoplasty was modified, and the modified labia minora flap vaginoplasty was employed to treat five patients with congenital absence of vagina.
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方法:00 年 9 月～00 年 9 月,对小阴唇瓣阴道成形术进行了改进,并采用改进的手术方法治疗先天性无阴道患者 例。 |
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Methods GHcDNA was cleaved by two kinds of restriction enzyme from pUC9-GHcDNA and inserted into eukaryotic expression vector pcDNA ,froming recombinant pcDNA -GH plastid. Its sequence was identified by cleaving of enzyme and sequencing.
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方法 用双酶切从 pUC9-GHcDNA中克隆出生长激素基因 ,然后定向插入真核细胞表达载体pcDNA 中 ,行酶切和测序以鉴定。 |
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Methods ICG(g·L - )was spred on the equatorial scleral surface of 0 Dutch belted eyes and 8 pig eyes. Another 8 pig eyes were not spred with ICG.
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方法 0只灰兔眼和 8只猪眼表面涂吲哚花青 (ICG)溶液 ,浓度 g· L- ,另 8只猪眼不涂 ICG溶液。 |
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Methods In this study,the BST-Ⅲbioshock tube was used to inflict ocular blast injury in rabbits. The peak pressure values for inflicting the mild and severe injuries were(9± 8.8)kPa(8.9±.8)ms and (8 ±.)kPa(.±.7)ms,respectively.
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方法 采用BST Ⅲ型生物激波管 ,建立以压力峰值( 9± 8.8)kPa和 (8 ± .)kPa的轻、重两组伤型的眼冲击伤模型。 |
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Methods In total inverted nipples, nipples were treated by double areolar flaps rotation, and 9 nipples were treated by Pitanguy′s method.
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方法 应用双瓣旋转法和改良Pitanguy法治疗重度乳头内陷共 侧 ,其中双瓣旋转法治疗 侧 ,改良Pitanguy法治疗 9侧。 |
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Methods New Zealand rabbits were immunized with synthetic Aβ peptide coupled to keyhole limpet haemocyanin (KLH) for the production of the polyclonal anti-Aβ antibody.
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方法 :将Aβ 多肽与匙孔虫戚血蓝蛋白 (KLH)偶联 ,免疫新西兰白兔从而产生抗 -Aβ 多克隆抗体。 |
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Methods Observe cellular membrane fluidity, cellular free calcium ion and cretin kinase(CK)in primary cellular culture under concentration of calcium in extracellular is .0 mmol/L、0.8mmol/L、0.mmol/L、0.mmol/L、0.mmol/L.
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方法观察细胞外钙离子浓度为．0mmol／L、0．8mmol／L、0．mmol／L、0．mmol／L、0．mmol／L时对原代培养心肌细胞膜流动性、细胞内游离钙和肌酸磷酸激酶（CK）的影响。 |
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