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Isolation、Identification and Sequence Analysis of Differential Expressed Genes Induced by IL-1β in NIT-1 Pancreatic β-Cells
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IL-1β诱导后NIT-1胰岛β细胞差异表达基因的分离、监定和序列分析 |
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It indicate that the insecticidal protein in the supernatant is thermal instable, which corresponding with the property of Vip3A protein.
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这说明上清蛋白含有一种对夜蛾科有高毒力和热不稳定性的蛋白，这与Vip3A蛋白的性质一致，从而确立了这种检测和鉴定方法的可行性。 |
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It is resulted that the percentage of supercoiled pUDKH in plasmid is more than 95%,and RNA undetected. The content of host protein remained is 0.0166 u g/mg and chDNA is less than 2ng/mg,endotoxin is 0.00178EU/mg.
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结果表明：质粒中超螺旋pUDKH含量达95％(质量百分比)以上，残留核糖核酸RNA检测不到，宿主蛋白含量小于10mg／mg质粒DNA，残留宿主DNA含量小于2ng／mg质粒DNA，内毒素含量约0.00178EU／mg。 |
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It is showed that the cell parameters increased with Zr~(4+)increasing and KTP octahedron crystal was badly distorted.
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随着掺杂Zr~(4+)浓度的增加,晶胞参数明显增大,KTP中八面体畸变的程度会更高。 |
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It means that the two genes, ETV5 and DKFZ, may be involved in the cell proliferation.
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推测ETV5、DKFZ两个基因可能与细胞的增殖相关。 |
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It showed BL21(DE3) was the better bacterial host. The method of pH-stat fed-batch culture was applied to obtain high cell density. The final cell density OD600 of grown pNS2/BL21(DE3) was 71.64 and the volumetric productivity was 0.8g/L.
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研究了重组质粒pNS2在两种宿主菌中的摇瓶与10L发酵罐培养表达情况，确定了较适的宿主菌为BL21(DE3)，采用pH-stat补料分批发酵，工程菌pNS2／BL21(DE3)最终菌体密度OD_(600)达71.64，表达量为0.8g／L。 |
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It showed that addition of ATP had an inhibitory effect on cell growth and did not increase 1,3-PD final concentration obviously, but increased 1,3-PD production per cell considerably. Addition of ATP can increase the yield of glycerol to 1,3-PD .
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添加ATP对菌体生长有抑制作用,对提高1,3-PD终浓度的作用不明显,但能明显促进单位细胞合成1,3-PD并提高甘油到1,3-PD的转化率。 |
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It was demonstrated that 83.33% specificity and 100% sensitivity in serum protein fingerprints clsssification and decision tree constructed by training samples.
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以28、60 d的对照组和染矽尘大鼠组为训练样本,血清蛋白指纹诊断决策树图分类敏感性和特异性分别达100%、83.33%。 |
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It was found that 57 genes increased and 5 genes decreased in the ischemic group, and 43 genes increased and 32 genes decreased in the Ad-HGF injection group.
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而Ad-HGF治疗后的上调基因与下调基因接近，43个基因表达降低，32个基因表达升高。 |
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It was found that POD enzyme activities in different parts of the plant were ordered as follow: mature leaves in the mid part(6.89×103U/g) >old leaves in the lower part(5.38×103U/g) >new leaves in the upper part(2.82×103U/g);
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结果表明:(1)同一植株不同部位的HLP酶活性大小顺序为,中部成熟叶(6.89×103U/g)>底部衰老叶(5.38×103U/g)>上部嫩叶(2.82×103U/g); |
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It was found that incidence of intrahepatocellular HBV DNA, HBsAg and HBcAg was 43.48% ,38.89% and 16.67% respectively, and 65.22% among the patients had HBV markers expression in the liver.
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发现肝内乙肝病毒脱氧核糖核酸(HBV DNA),HBsAg,HBcAg的检出率分别为43.48％、38.89％. 16.67％; |
