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The tumor cell vaccine (TCV-m4-1BBL) was obtained by treating Hepa1-6-m4-1BBL with mitomycin (MMC). Cocultivation TCV with syngeneic murine spleen cells and the supernatants were harvested for detecting the cytokines (IL-2,TNF-α and GM-CSF).
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方法以丝裂霉素C(MMC)处理高表达转m4-1BBL基因的小鼠Hepa1-6肝癌细胞,制成肿瘤细胞瘤苗(TCV),体外与同系小鼠脾淋巴细胞共同培养后,观察其对脾细胞产生细胞因子(IL-2、TNF-α和GM-CSF)的影响。 |
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The tumor specific CTL was generated form activated autologous T cell by DC vaccine loaded with tumor antigen, and the killing activity of CTL to CT26 cells was tested by the lactate dehydrogenase (LDH) release assay in vitro.
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负载肿瘤抗原的DC疫苗激活同源T淋巴细胞产生细胞毒性T淋巴细胞(CTL),采用乳酸脱氢酶(LDH)4h释放法检测CTL在体外对CT26细胞的杀伤活性; |
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The tumors were predominate B cell lymphoma (5/7 cases)and less common in T cell(1/7 cases)and histiocyte lymphoma(1/7 cases).
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多数为B细胞性淋巴瘤 ( 5 7例 ) ,T细胞性淋巴瘤 ( 1 7例 )和组织细胞性淋巴瘤少见 ( 1 7例 )。 |
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The tumorswere predominate B cells lymphere(5/7 cases), occasional T cell 1/7 cases) and histiocyte lymphoma (1/7 cases).
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多数为B细胞性淋巴瘤（5／7例），T细胞性淋巴瘤（1/7例）和组织细胞性淋巴瘤（1／7例）少见。 |
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The ultrastructure of human mesenchymal stem cells
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人骨髓间充质干细胞的超微结构 |
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The use of joint imaging with 99m Tc labeled human immunoglobulin G in rheumatoid arthritis
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~(99m)Tc标记人免疫球蛋白关节显像在类风湿关节炎中的应用 |
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The valence electron structures of the segregated cell of Al-Li alloy in earlier aging condition are calculated according to the empirical electron theory (EET) in solid.
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运用固体经验电子理论(EET),对Al-Li合金时效初期的偏聚晶胞的价电子结构进行计算。 |
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The vascularized nerve was transferred to the lateral abdominal wall from a subcutaneous tunnel and anastomosed to the excised ilioinguinal nerve and lateral cutaneous nerve of thigh in the distal end by sura nerve graft.
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切取截瘫平面以上1～2个节段的肋间神经外侧皮支（一般为第7／8或8／9肋间神经），带肋间动静脉转位经皮下隧道引至腹壁外侧，与切断的髂腹股沟神经和股外侧皮神经远端用移植的腓肠神经桥接吻合。 |
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The wet cell output reached 30 g/L and rhALR expression quantity was 30%.
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最终菌体湿重达到30g／L以上，rhALR表达量为30％。 |
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The wheat Rosette Stunt Virus(WRSV)RNA could be isolated by treatment of the intact virus or viral nucleocapsid with SDS-Urea and water-saturated phenol.
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小麦丛矮病毒(Wheat Rosette Stunt Virus.以下简称WRSV)的核酸可以通过SDS-尿素处理和苯酚抽提的方法分离获得。 |
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The work of this dissertation can be summarized as follows:In the first part of the dissertation, we study NEFCLASS (NEuro Fuzzy CLASSification), a neuro-fuzzy system for the data classification.
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第一部分研究了通过调整隶属函数的参数来学习的神经模糊分类器NEFCLASS(NEuro Fuzzy CLASSification)。 |
