|
| Methods Amplify Rpf E gene from the genomic DNA of M.tuberculosis H37Rv by PCR,insert into expression vector pET32a(+) and transform to E. |
中文意思：
方法采用PCR方法从结核分枝杆菌H37Rv基因组DNA中扩增出RpfE基因,双内切酶消化后,与同样酶消化的pET32a(+)载体连接,转化大肠杆菌Top10。 |
以下为相关句子列表(点击查看，可以看对应的解释）：
|
|
Methods 5 cases of lymphoblastic NK/T cell lymphomas were collected ,4 cases from lymph nodes and 1 case from nasal cavity, the nature of neoplastic cells was confirmed by immunohistochemistry.
|
|
|
方法 收集 5例母细胞性NK/T细胞淋巴瘤 ,1例原发于鼻腔 ,4例原发于淋巴结。 |
|
Methods 84 STD clinic patients' genitourinary tract secretions were collected for Mg DNA detection by polymerase chain reaction (PCR).
|
|
|
方法采集84例性病门诊患者泌尿生殖道分泌物,用聚合酶链反应(PCR)技术检测生殖支原体脱氧核糖核酸(MgDNA)。 |
|
Methods 87 patients aged from 11 month to 13 years were randomly divided into a treatment group (57 cases) and a control group (30 cases). The treatment group was administrated with Hunan immunoglobulin injection. <2y 300mg/d, 3-6y 600mg/d.
|
|
|
方法87例11个月～13岁的本科住院患儿随机分为治疗组:57例,对照组:30例,治疗组应用国产人血丙种球蛋白注射液,<2岁300mg/d,3～6岁600mg/d,>7岁600～1200mg/d,肌肉注射,1～2次/d,疗程5～7天。 |
|
Methods A proband and his 5 family members with T53→C in intron 1 and C59→A in intron 4 mutations of ApoM gene expression and their lipids were detected by polymerase chain reaction(PCR) direct DNA sequencing in a Han nationality Chinese family.
|
|
|
方法应用聚合酶链反应DNA直接测序法,检测了1个携带ApoM基因1号内含子T53→C、4号内含子C59→A突变的先证者及其5名家系成员这2个位点的基因型及血脂情况。 |
|
Methods A set of universal primers was designed based on the conservative regions in bacterial 16S ribosomal RNA genes. 7 control bacterial strains,human DNA,HBV-DNA and 37 samples of ascites of patients with cirrhosis were amplified with PCR.
|
|
|
方法在细菌16S rRNA基因保守区设计一对通用引物,对7种对照菌株、人基因组DNA、HBV-DNA、37份肝硬化患者腹水进行PCR扩增。 |
|
Methods Amplify Rpf E gene from the genomic DNA of M.tuberculosis H37Rv by PCR,insert into expression vector pET32a(+) and transform to E.
|
|
|
方法采用PCR方法从结核分枝杆菌H37Rv基因组DNA中扩增出RpfE基因,双内切酶消化后,与同样酶消化的pET32a(+)载体连接,转化大肠杆菌Top10。 |
|
Methods Analyze and compare the most successful models,cell automaton and car-following model,and point out their advantage and shortcomings.
|
|
|
方法对现有比较成功的微观模型、元胞自动机模型和跟驰模型进行分析、比较,指出其不足和优点。 |
|
Methods BALB/c mice were subcutaneously injected with 0.8mg of the synthesized peptide to produce the experimental autoimmune myocarditis (EAM) models.
|
|
|
方法:人工合成含心肌肌球蛋白抗原重链表位长度为30个氨基酸残基的多肽0.8mg,纯化多肽皮下免疫遗传易感BALB/c小鼠,建立自身免疫性心肌炎模型,检测TNF-α和cTnI病变期间的改变。 |
|
Methods Base on MIC(morphology,immunology and cytogenetics),nested reverse transiption-polymerase chain reaction(RT-PCR) was performed to detect TEL-AML1 fusion gene.
|
|
|
方法在MIC分型(形态学、免疫学、细胞遗传学)基础上,采用巢式逆转录-聚合酶链反应(RT-PCR)检测ALL患儿90例TEL-AML1融合基因。 |
|
Methods CD117~+ hemopoietic stem cells (HSCs) were isolated and purified from the bone marrow of healthy C57 mice by magnetic affinity cell sorting.
|
|
|
方法取健康C57小鼠骨髓,通过MACS系统分离、纯化CD117~+造血干细胞; |
|
Methods Collecting 106 698 data from Medical Examination Center of Shenzhen People's Hospital from 2003 to 2005,and analyzing liver function,blood fat,blood sugar,Hepsati-B antigon,tumor index according to different age groups.
|
|
|
方法:收集2003年至2005年在深圳市人民医院体检中心体检人员的资料106698例,按年龄分组对肝功能、血脂、血糖、乙型肝炎表面抗原(HBsAg)、肿瘤标志物的实验室结果进行分析。 |
|
|
|
