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Methods:The mouse 11beta-HSD1siRNA plasmids were transfected into 3T3-L1 cells for the examination of the effect of targeted 11beta-HSD1 inhibition on differentiation of 3T3-L1 cells.
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方法:构建11β-HSD1-SiRNA表达质粒pGCsilencerTMH1/TetO1-11β-HSD1并稳定转染3T3-L1细胞,采用油红染色观察脂滴堆积情况,采用Westernblot方法检测11β-HSD1在正常3T3-L1细胞分化过程中的表达; |
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Methods:The result of electrophoresis,purity and enzyme digesting of genome DNA of three kind of Pinaceae were compared.
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方法:分别比较了用传统的CTAB法、SDS法以及3种改良的CTAB法提取的上述3种松科植物叶片基因组DNA的电泳、纯度和酶切效果。 |
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Methods:The-455nt polymorphisms in the βfibrinogen gene were detected by polymerase chair reaction amplication of fragments of the DNA.
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方法β纤维蛋白原基因-455nt多态性分析:(1)碘化钾法提取人基因组DNA; |
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Methods:We constructed a partial double-strand DNA with T7 promoter as DNA template and synthesized small hairpinRNA in-vitro using T7 RNA polymerase.
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方法：根据端粒酶hTERT基因1573—1591位的核酸序列，构建带T7启动子的部分双链DNA模板，用T7RNA聚合酶体外合成短链shRNA。 |
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MethodsITP children,102 cases,were divided into two groups: HBV-ITP group(25 cases) and non-HBV-ITP group(77). All the children were given high dose intravenous immunoglobulin.
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方法将102例ITP病儿,分为两组,其中HBV-ITP组25例,非HBV-ITP组77例,病儿均采用大剂量丙种球蛋白、常规治疗量泼尼松、小剂量长春新碱等治疗,比较两组病儿的临床特征、治疗反应、病程特点等。 |
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MethodsSerum MCP-1,TNFαand IL-6 were detected by radioimmunoassay and enzyme linked immunosorbent assay(ELISA)4 weeks before and after oral administration of pravastatin in 25 patients with UA.
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方法采用放射免疫法及酶联免疫吸附法(ELISA)分别检测了25例UA患者服用普伐他汀前后血清MCP-1、TNFα及IL-6水平。 |
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MethodsSome reaction condition was optimized by amplifying technology and method of PCR based on isolating genomic DNA from D.
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方法在提取纯化美花石斛基因组DNA的基础上,利用PCR扩增技术和方法,对Mg2+,dNTP,模板DNA,引物,Taq聚合酶等反应条件进行优化。 |
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MethodsSpecific antibodies and DNA to HSV-1 were measured in 51 patients with AMI(AMI group),42 patients with old myocardial infarction (OMI group) and 31 normal controls (NC group) who had no angiographic evidence of coronary artery disease.
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方法测定51例急性心肌梗死(AMI)和42例陈旧性心肌梗死(OMI)患者及31例冠脉造影正常者(NC)的HSV1抗体水平及脱氧核糖核酸(DNA),同时观测纤维蛋白原(Fg)、6酮前列环素F1α(6Keto PGF1a)、血栓素B2(TXB2)、P选择素(GMP140)变化。 |
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Methylation of Genomic DNA and Proto-oncogene c-Ha-ras in Brain Nuclei of Ageing Rats
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大鼠衰老过程中脑细胞DNA与c－Ha－ras原癌基因的甲基化 |
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Microbiology Resources in Internet and its Application in Multimedia Teaching Technology
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微生物学网络资源在多媒体教学中的应用 |
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Microbiology Teaching and Fostering Scientific Quality of the Student in Teachers Colleges
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高师微生物学教学与学生科学素质的培养初探 |
