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MethodsSerum MCP-1,TNFαand IL-6 were detected by radioimmunoassay and enzyme linked immunosorbent assay(ELISA)4 weeks before and after oral administration of pravastatin in 25 patients with UA.
中文意思:
方法采用放射免疫法及酶联免疫吸附法(ELISA)分别检测了25例UA患者服用普伐他汀前后血清MCP-1、TNFα及IL-6水平。


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Methods:The mouse 11beta-HSD1siRNA plasmids were transfected into 3T3-L1 cells for the examination of the effect of targeted 11beta-HSD1 inhibition on differentiation of 3T3-L1 cells. 方法:构建11β-HSD1-SiRNA表达质粒pGCsilencerTMH1/TetO1-11β-HSD1并稳定转染3T3-L1细胞,采用油红染色观察脂滴堆积情况,采用Westernblot方法检测11β-HSD1在正常3T3-L1细胞分化过程中的表达;
Methods:The result of electrophoresis,purity and enzyme digesting of genome DNA of three kind of Pinaceae were compared. 方法:分别比较了用传统的CTAB法、SDS法以及3种改良的CTAB法提取的上述3种松科植物叶片基因组DNA的电泳、纯度和酶切效果。
Methods:The-455nt polymorphisms in the βfibrinogen gene were detected by polymerase chair reaction amplication of fragments of the DNA. 方法β纤维蛋白原基因-455nt多态性分析:(1)碘化钾法提取人基因组DNA;
Methods:We constructed a partial double-strand DNA with T7 promoter as DNA template and synthesized small hairpinRNA in-vitro using T7 RNA polymerase. 方法:根据端粒酶hTERT基因1573—1591位的核酸序列,构建带T7启动子的部分双链DNA模板,用T7RNA聚合酶体外合成短链shRNA。
MethodsITP children,102 cases,were divided into two groups: HBV-ITP group(25 cases) and non-HBV-ITP group(77). All the children were given high dose intravenous immunoglobulin. 方法将102例ITP病儿,分为两组,其中HBV-ITP组25例,非HBV-ITP组77例,病儿均采用大剂量丙种球蛋白、常规治疗量泼尼松、小剂量长春新碱等治疗,比较两组病儿的临床特征、治疗反应、病程特点等。
MethodsSerum MCP-1,TNFαand IL-6 were detected by radioimmunoassay and enzyme linked immunosorbent assay(ELISA)4 weeks before and after oral administration of pravastatin in 25 patients with UA. 方法采用放射免疫法及酶联免疫吸附法(ELISA)分别检测了25例UA患者服用普伐他汀前后血清MCP-1、TNFα及IL-6水平。
MethodsSome reaction condition was optimized by amplifying technology and method of PCR based on isolating genomic DNA from D. 方法在提取纯化美花石斛基因组DNA的基础上,利用PCR扩增技术和方法,对Mg2+,dNTP,模板DNA,引物,Taq聚合酶等反应条件进行优化。
MethodsSpecific antibodies and DNA to HSV-1 were measured in 51 patients with AMI(AMI group),42 patients with old myocardial infarction (OMI group) and 31 normal controls (NC group) who had no angiographic evidence of coronary artery disease. 方法测定51例急性心肌梗死(AMI)和42例陈旧性心肌梗死(OMI)患者及31例冠脉造影正常者(NC)的HSV1抗体水平及脱氧核糖核酸(DNA),同时观测纤维蛋白原(Fg)、6酮前列环素F1α(6Keto PGF1a)、血栓素B2(TXB2)、P选择素(GMP140)变化。
Methylation of Genomic DNA and Proto-oncogene c-Ha-ras in Brain Nuclei of Ageing Rats 大鼠衰老过程中脑细胞DNA与c-Ha-ras原癌基因的甲基化
Microbiology Resources in Internet and its Application in Multimedia Teaching Technology 微生物学网络资源在多媒体教学中的应用
Microbiology Teaching and Fostering Scientific Quality of the Student in Teachers Colleges 高师微生物学教学与学生科学素质的培养初探
 
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