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Developmental toxicity in zebrafish embryos after exposure to three fullerene aggregates (nC_(60)) and fullerol;
中文意思:
富勒烯及其衍生物对斑马鱼胚胎发育毒性的比较


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0 kb 5′arm (long arm,LA) of rat HPRT gene knock-out vector was cut by SalⅠ from rat HPRT gene genomic bacterial artificial chromosome (BAC),and the 1. 根据已知大鼠次黄嘌呤鸟嘌呤磷酸核糖转移酶 (HypoxanthineGuaninePhosphoribosylTransferase ,HPRT)基因的外显子序列 ,从大鼠HPRT基因组DNA序列的细菌人工染色体 (BacterialArtificialChromosome ,BAC)中用酶切和PCR方法分别分离得到用于构建基因敲除载体的 3 0kb的 5′长臂 (LongArm ,LA)和 1 7kb的 3′短臂 (ShortArm ,SA) ,并分别克隆到pSL1180和pCR2 1中。
7kb exon6 to exon7 DNA sequence which is separated by PCR as Short Arm(SA),were used to construct HPRT gene knock out vector. 根据已知大鼠HPRT基因的外显子序列,从大鼠HPRT基因BAC中用酶切和PCR方法分别分离得到用于构建基因敲除载体的3。
As the blind students have the characteristics of hearing,feeling and memory supernormal,the teaching ways of making best use of the advantages and bypassing the disadvantages,and oral instruction and heart conduction are adopted. 根据盲人学生听觉、触觉、记忆超常的特点 ,采取扬长避短 ,口传心授的教学方法 ,取得了良好的教学效果。
The Study on Cooperation Theory of Hearing, Feeling and Seeing in Piano Teaching; 钢琴演奏教学中听觉、触觉、视觉的协同研究
The Study on Cooperation Theory of Hearing, Feeling and Seeing in Piano Teaching; 钢琴演奏教学中听觉、触觉、视觉的协同研究
Developmental toxicity in zebrafish embryos after exposure to three fullerene aggregates (nC_(60)) and fullerol; 富勒烯及其衍生物对斑马鱼胚胎发育毒性的比较
A range of zebrafish(Danio rerio) were exposed to 3-methylcholanthrene(3-MC) by water pollution at the doses of 25 μg/L,50 μg/L,100 μg/L,200 μg/L and 400 μg/L,respectively. 分别用25、50、100、200和400μg/L的3-甲基胆蒽(3-MC)对斑马鱼进行水浴染毒,于第7天和第14天采集各染毒组和对照组活鱼的肝、鳃、心、肠和肾,进行病理组织学检查。
Effects of phenanthrene toxicity on histopathology of Brachydanio rerio gill and liver; 菲对斑马鱼鳃和肝组织结构的影响
M ethods Whole embryo culture system was used in this research. 方法采用植入后全胚胎培养模型,将9。
Methods Whole embryo culture system was used in this research. 方法采用植入后全胚胎培养模型,将8。
Methods The post-implantation whole embryo culture (WEC) system was used to observe the effects of Lead on cultured mouse embryo in vitro. 方法 采用全胚胎培养技术 ,将孕 8。
 
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