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METHODS 110 batches of FFP, human immunoglobulin and human serum albumin were detected for anti-HCV, HCV RNA, anti-HIV (1+2) 、HIV-1RNA by ELISA, western blot (WB) and PCR.
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方法用ELISA、WB和PCR对110批FFP、人血丙种球蛋白、人血白蛋白检测了抗HCV、HCVRNA、抗HIV（1＋2）、HIV－1RNA。 |
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METHODS the pharmacokinetics of EPC and HEPC sterically stabilized liposomes (EPC-SSL, and HEPC-SSL) were studied by HPLC.
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方法用高效液相色谱法研究EPC和HEPC长循环阿霉素脂质体在大鼠体内的药物动力学。 |
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METHODS: Reverse transcription polymerase chain reaction (RT-PCR) technique was used to detect the expression of β-G mRNA in 10 cases of normal liver tissue and 38 cases of different differentiation tissues of hepatocellular carcinoma.
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方法采用逆转录-聚合酶链反应(RT-PCR)方法,对10例正常肝脏及38例不同分化程度肝癌组织中β-G mRNA的表达进行对比研究。 |
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METHODS: The experiment was performed in the Pharmacology Laboratory of Jinzhou Medical College from October 2005 to May 2006. Fifty SD rats were randomly divided into five groups: sham, model, ZG groups of 167, 250 and 357 mg/kg.
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方法:实验于2005-10/2006-05在锦州医学院药理学实验室进行,50只SD大鼠随机分为5组,即假手术组,缺血再灌注模型组,葡萄糖酸锌167,250,357mg/kg剂量组。 |
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METHODS: The pcDNA3.1-FKBP12.6 plasmid, mingled with albumin-coated microbubbles agents, was transfected into H9c2 (2-1) cells by ultrasound-mediated destruction of microbubbles. The H9c2 (2-1) cell growth state was investigated by inverted microscope.
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方法:将pcDNA3.1-FKBP12.6质粒与白蛋白包裹微泡造影剂混合,经超声转染H9c2(2-1)细胞后,通过倒置显微镜观察心肌细胞生长状况的变化; |
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METHODS: The synthesis of DNA and collagen was determined by measuring the incorporation of [3H]TdR and [3H]proline of HLF respectively.
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方法:采用[~3H]TdR与[~3H]脯氨酸掺入分别测定脱氧核糖核酸和胶原合成。 |
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METHODS: To observe the effect of propafenone, we gave various concentration gradient (1, 6, 10 μmol/L) on the APD of endocardium myocytes in ischemic state with whole cell patch-clamp through alterring frequency of stimulation.
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方法采用全细胞膜片钳技术,观察在不同刺激频率(即基础循环周长,BCL=2000、1000、500、250ms)下,不同浓度(1、6、10μmol/L)的普罗帕酮对缺血心肌左右心室心内膜心肌细胞动作电位时程(APD)的影响。 |
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METHODS: healthy malevolunteers (age 22.7 ±0.7 years old,rang 22-24 years old, weight 60.6 ±4.5kg)werestudied in order to understand the pharmacokinetics of GFA.
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为研究单次静脉注射盐酸GFA人体内药代动力学。 方法：健康男性10名，年龄：22岁－24岁（平均22．7±0．7岁）； |
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METHODS: ① The experiment was done in the laboratory of molecular immunology, Weifang Medical College from October 2002 to October 2003. Thirty healthy male Wistar rats aged 8 weeks old were selected.
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方法：①实验于2002-10／2003-10在潍坊医学院分子免疫实验室完成。 选用30只8周龄健康雄性Wistar大鼠。 |
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METHODS:Gene expression of human FL cells treated with 0.2 μmol/L MNNG was detected by high throughput real-time PCR.
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方法:用ABI公司的高通量实时荧光定量PCR方法,检测FL细胞在0.2μmol/LMNNG处理后基因表达发生的改变。 |
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METHODS:Pharmacology, clinical usage and ADR of digitalis were analyzed and evaluated.
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方法 :从洋地黄类药物的药理作用、临床应用及其不良反应方面进行分析、评价。 |
