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A proteinase K digestion and phenol-chloroform extraction method was used to extract IBDV dsRNA from bursae of infected chicken. The VP gene was amplified by RT-PCR, and then cloned into pMD8-T vector for sequence analysis.
中文意思:
用蛋白酶K消化、酚-氯仿抽提法成功提取出IBDV基因组RNA,用RT-PCR技术扩增出IBDV的结构蛋白基因VP,并将其克隆到pMD8-T载体。


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A preliminary results were reported on the effects of penicillinum G natricum on the induction and differentiation of Spathiphyllum Kochii’s callus tissue,and on its seedling growth. 报道了青霉素G钠对绿巨人愈伤组织诱导、分化及苗生长的初步结果 .
A preliminary study of Chloroph-orus diadema Motschulsky. 刺槐绿虎天牛的初步研究
A preliminary survey of gadfly insects at Dawenliu grassland 大汶流草场虻类昆虫种类初步调查
A probe into the social background of new Olympic mottos creating 对奥林匹克新格言产生的社会背景之探讨
A progress study on the relationship between postmortem interval and the changes of DNA content in the heart cellule of rat 大鼠死后心肌细胞DNA含量与死亡时间关系的进一步研究
A proteinase K digestion and phenol-chloroform extraction method was used to extract IBDV dsRNA from bursae of infected chicken. The VP gene was amplified by RT-PCR, and then cloned into pMD8-T vector for sequence analysis. 用蛋白酶K消化、酚-氯仿抽提法成功提取出IBDV基因组RNA,用RT-PCR技术扩增出IBDV的结构蛋白基因VP,并将其克隆到pMD8-T载体。
A randomised controlled trial on photo(chemo)therapy of subacute prurigo 光化学疗法治疗亚急性痒疹的一项随机对照试验
A rare case of bullous morphea(BM) was reported. 报告 例少见的大疱性硬斑病。
A rare case of nasal and buccal obstruction in a child: The antrochoanal polyp of Killian (Fren) 例罕见的鼻和口腔阻塞的儿童病例:鼻窦后鼻孔Killan息肉
A rational categorizing method is suggested, and the design and the characteristics of the velours are expounded in this paper. 本文对此进行了合理的分类,并论述了各类经编绒类织物的设计和结构特征。
A s a result of conversion,molar combustion heat of castor oil at constant pressur e is - 8.kJ·mol-,standard molar formation enthalpy is -8. kJ·mol - 经恒压燃烧热换算与标准生成焓转换得到蓖麻油的恒压摩尔燃烧热为- 8.kJ/mol,标准摩尔生成焓为-8.kJ/mol。
 
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