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Methods Test the activity of CD69 and level of IL-2 by cell flow cytometer after colon cancer operation in 33 cases.
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方法应用流式细胞仪检测33例结肠癌患者手术前后外周血中T淋巴细胞CD69的表达百分率及IL-2的表达水平,并与对照组进行比较分析。 |
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Methods The HBV DNA in HepG 2.2.15 cell line supernatant with/without IFNα-2b were monitored by fluorescence real-time quantitive PCR.
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方法 用定量聚合酶链反应(PCR)检测IFNα2b处理前、后的HepG2 2 15细胞上清的乙型肝炎病毒脱氧核糖核酸(HBVDNA)水平。 |
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Methods The MK levels in serum were determined with enzyme-linked immunosorbent assays for 20 healthy women,20 patients with breast benign diseases and 64 patients with breast cancer.
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方法采用酶联免疫吸附法对20例正常女性,20例女性乳腺良性疾病和64例乳腺癌患者血清MK进行检测。 |
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Methods The TK6 cell was cultured and its genomic DNA was extracted and prepared to the single-stranded probes of the exon 2 of k-ras gene.
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方法培养TK6细胞,抽提基因组DNA,制备k-ras基因外显子2的单链探针。 |
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Methods The allergen of inhalation and edibility in 311 cases with allergic dermatitis and 30 healthy persons were detected by enzyme-linked immunosorbent assay (ELISA).
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方法采用酶联免疫吸附试验(ELISA)对311例过敏性皮肤病患者和30名健康对照者血清中吸入性及食入性过敏原进行检测。 |
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Methods The effects of dopamine (DA) and glutathione (GSH) on apoptotic PC12 cell were examined by fluorescence microscopy using the DNA end- labeling method.
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方法用脱氧核糖核酸（DNA）标记法，借助荧光显微镜观察多巴胺（DA）和还原型谷胱甘肽（GSH）对PC12细胞凋亡的影响。 |
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Methods The expression levels of the P53 protein and Ki-67 were examined by immunohistochemistry(S-P method). To analysis the relationship between levels of P53 protein and Ki-67 and differentiation,clinical stage and metastasis of esophageal carcinoma.
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方法应用免疫化学S-P法对50例食管鳞状细胞癌中P53蛋白和Ki-67的表达水平进行研究,分析其与食管癌组织分化程度、临床分期及转移的关系。 |
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Methods The heparinaseⅡ gene was amplified with PCR from the genomic DNA of Flavobacterium heparinum, and it was linked into expression plasmid pET-28a after sequencing, then the recombined plasmid was expressed in E.
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方法通过PCR从黄杆菌基因组DNA中扩增出黄杆菌HepⅡ的基因,经验证后插入到表达质粒pET-28a上构建表达载体,重组质粒转化E. |
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Methods The level of Th1 and Th2 cytokines in serum were detected by flow fluorenscence immunmicrobeads assay (FFIA) from 44 patients with breast cancer and 47 cases of patients with breast benign Tumour.
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方法用流式荧光免疫微球分析技术(Flow Fluorenscence Immunmicrobeads Assay,FFIA)检测乳腺良、恶性肿瘤患者血清Th1/Th2细胞因子水平。 |
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Methods The samples of serum and BALF were obtained from 47 patients with untreated primary lung cancer and 18 patients with benign pulmonary diseases. The levels of endostatin were analyzed by enzyme-linked immunosorbent assay(ELISA).
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方法采用酶联免疫吸附法(En-zyme-linked immunosorbent assay,ELISA)检测初诊肺癌47例及肺良性病变18例患者外周血清及BALF中Endostatin的表达水平。 |
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Methods The serum anti-β_2GPI and anti-cardiolipin antibodies(ACA) were measured by enzyme-linked immunosorbent assay(ELISA) in 56 patients with SLE and 40 healthy controls.
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方法采用酶联免疫吸附试验(ELISA)半定量测定56例SLE患者及40名健康对照者血清抗β2GPI和抗心磷脂抗体(ACA)阳性率。 |
