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Methods CD8 gene was cut out from plasmid pCDM8 by restriction endonuclease Xho Ⅰ. It was inserted into eukaryotic expression vector pCDNA and identified by restriction enzyme BamH Ⅰ.
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方法 用限制性内切酶XhoⅠ酶切质粒pCDM8得到CD8基因 ,并将其插入pCDNA ,用BamHⅠ酶切鉴定。 |
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Methods CFW in megathermal(atmospheric temperature, 9℃±0.℃) and megahumid environment(relative humidity,80%～8%) was made by pistol in 0 dogs. The dogs with CFW were randomly divided averagely into hypothermia and control groups.
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方法选杂种犬0只,制作出高温高湿环境下(气温 9℃±0.℃,相对湿度80%～8%)颅脑火器伤的动物模型,随机分为亚低温组和对照组,每组 0只犬。 |
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Methods CT findings of cases of urachal abnormalities proven by surgery and pathology were retrospectively reviewed, the emphasis was focused on its location, shape, size, enhancement-pattern and radiological features in Retzius space.
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方法回顾分析 例经手术病理证实的脐尿管病变的CT表现,包括病灶部位、形态、大小、增强以及Retzius间隙影像改变。 |
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Methods Checklist on Quality of Life issued by World Health Organization (WHOQOL-BRIEF) and Social Support Rating (SSRS) were used to conduct a survey in 8 discharged patients suffering from traumatic paraplegia.
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方法采用世界卫生组织生存质量测定量表简表(WHOQOL-BRIEF)和社会支持评定量表(SSRS)对8例外伤性截瘫患者在出院后进行追踪调查,并对其生存质量与社会支持关系进行相关性分析。 |
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Methods Chest CT images in cases of pectus excavatum from 00-0 —00-07 in our hospital were retrospectively analysed.
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方法对我院00-0 —00-07间经手术治疗的 例患漏斗胸的患儿胸部CT检查进行回顾性的分析。 |
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Methods Clinical symptom and laboratory test were analyzed of 0 patients with Eosinophilic prurigo(A group) and 0 patients with Prurigo simplex(B group).
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方法对嗜酸性痒疹(A组) 0例、寻常性痒疹(B组) 0例的临床症状及实验室检查等资料进行对比分析。 |
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Methods DNA contents in coelom fluid cells were measured and analyzed from 9 patients with malignant carcinomas by flow cytometry.
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方法 采用流式细胞术对尚未进行化疗的9例恶性肿瘤患者体腔液细胞DNA含量进行了检测和分析。 |
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Methods DNA was extracted from 0 samples gathered respectively from decomposed tissue,fingernail(toenail) and hair stem treated with Chelex-00,Chelex-00 containing dithiothreitol(DTT) and the polymerse chain reaction(PCR) buffer.
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方法收集腐败肌肉、指(趾)甲及毛干各0份,采用Chelex-00法、含有DTT的Chelex-00处理法及PCR缓冲液处理法三种方法提取DNA,经PCR扩增,PAG电泳分离、银染技术进行mtDNA(CA)n重复子分型。 |
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Methods Early somite stage (GD 8.) mouse embryos were explanted and cultured in rat serum medium (immediately centrifugal serum,ICS) with atRA(0,0.0,0.,0.,0.,.0,0.0 μmol/L) for 8 h to evaluate the embryo development and morphological differentiation.
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方法孕8.日龄小鼠胚胎移植于atRA终浓度为0、0.0、0.、0.、0.、.0、0.0μmol/L的全胚胎培养基中,体外连续培养8h,观察atRA对胚胎发育和器官形态分化的影响。 |
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Methods Emodin was separated on Lichrospher C8(.×00mm,μm)with a mobile phase of methanol-0.% phosphoric acid(8∶) and detected at 90nm with a flow rate of .0(ml/min).
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方法色谱柱:L ichrospher C8(.×00mm,μm),流动相:甲醇-0.%磷酸溶液(8∶),流速.0 m l/m in,检测波长90nm。 |
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Methods Expression of TLR and TLR was measured in specimens from lesions of guttate psoriasis, from chronic plaque psoriasis and 0 from normal skin.
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方法选用例滴状银屑病、 例斑块状银屑病患者及0例正常人皮肤的石蜡切片,用免疫组化的方法研究TLR和TLR的表达。 |
