|
16SrRNA gene of ten bacterial species were amplified with PCR,by using human genome DNA、HBV-DNA and candida albicans as comparison;
|
|
|
用PCR技术扩增实验室保留株10株的16SrRNA基因,以人类基因组DNA、HBV-DNA和白色念珠菌为对照,检测该方法的特异性; |
|
2) TCS could increase the systematic production of Th2 cytokines IL-4 toward the shift of Th1/Th2 balance.
|
|
|
CD4+T前体细胞向ThZ细胞转化。 2)增加ThZ型细胞因子IL一4的全身表达，从而有效调节Thl/Th2 |
|
2. Combined the routine methods applied to immunology and molecular biology, IVIAT is a rapid and convenient new way which can be used to study the in vivo induced genes of pathogens.
|
|
|
2.IVIAT是一个崭新的研究体内表达基因的方法，快速、简便，无须使用动物模型，仅使用常规的免疫学和分子生物学实验方法，是研究病原微生物体内诱导基因的一个很有价值的手段。 |
|
2. Exogenous ceramide can in certain extent increase the cell's free Ca2+ as well as induce the NIH 3T3 cell apoptosis, increased was mainly come from the extracellular cadium initiated by cadium exhausting.
|
|
|
2.外源性神经酰胺在一定的剂量范围内可以导致NIH3T3细胞的凋亡和细胞内游离钙的升高并且有一定的剂量依赖性关系,这说明Ca~(2+)参与了凋亡信号的传递; 胞内游离钙的增加主要来源于钙池耗竭引发的外Ca~(2+)内流; |
|
2. The concentration–time data of after oral administration in healthy volunteers were best fitted to two-compartment model. The main pharmacokinetics parameters were as follow: Cmax were (947.16±174.98)、(758.86±193.53) and (938.71±202.37) μg·L-1;
|
|
|
(2)健康志愿者口服环孢素A胶囊后,血药浓度时间-曲线符合二室模型,测得参比制剂新山地明胶囊及两种参比制剂因普兰他胶囊和田可胶囊药动学参数:Cmax分别为(947.16±174.98)、(758.86±193.53)和(938.71±202.37)μg·L-1; |
|
2. There exist the distinction of not only the percentage of Th and Tc cells, but also the production cytokines ability of T lymphocyte.
|
|
|
2.CA患者不但存在着Th、Tc细胞数量上的差异，从平均荧光强度分析可以看出，还存在着T细胞分泌细胞因子能力的改变，分泌Thl型细胞因子能力下降，而分泌ThZ型细胞因子的能力增高。 |
|
2. epirubicin and RNA , such as RNA Type Ⅲ, RNA Type Ⅵ systems;
|
|
|
2、RNA Type Ⅲ、RNA Type Ⅵ等核糖核酸与葸环类抗生素表柔比星(EPI)相互作用的反应体系； |
|
23 elderly patients with multiple osseous metastases of cancer were treated with 153Sm-EDTMP, and pharmacokinetics studied.
|
|
|
应用~(153)Sm-EDTMP治疗老年骨转移癌患者23例,并进行患者体内药代动力学研究。 |
|
24 polymorphic primer pairs were selected from the 27 primer pairs by using the genomic DNA of 27 sweet cherry germplasm,and 24 locus-specific SSR markers were obtained.
|
|
|
最后,以27个甜樱桃种质的基因组DNA为模板,从27对可扩增出带的引物中,筛选出多态性引物24对,获得了24个甜樱桃基因座特异性SSR标记。 |
|
25(86%)cases underwent radical resection,4 (14%)cases of palliative resection. The overall postoperative complication rate was 13.7% (4/29). The liver function was recovered within 10 d;
|
|
|
行根治性切除25例(86%),姑息性切除4例(14%),并发症发生率13.7%(4/29),术后肝功能10d恢复正常。 |
|
3. Combining the in silico cloning, RACE and RT-PCR method, the full-length cDNA sequence of PSMC1, PSMC4 and PSMC5 gene and the entire coding region of the PSMC2 and PSMD4 genes were cloned.
|
|
|
3．利用电脑克隆、RACE和RT-PCR的方法相结合，克隆了蛋白酶体激活因子PA700相关基因PSMC1、PSMC4和PSMC5共3个基因的全长cDNA序列，以及PSMC2和PSMD4基因的完整编码区(coding sequence，CDS)。 进行了蛋白质序列的推导和功能域的预测。 |
