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METHOD Human umbilicus vein endothelial cell and lipopolysaccharide were used to establish apoptosis cell models,and flow cytometer and confocal microscope were used to observe the effect of the medicated serum on ICAM-1 expression and intracellular Ca2+.
中文意思:
方法取人脐静脉内皮细胞(HUVEC),用脂多糖造成凋亡细胞模型,通过流式细胞仪、激光共聚焦显微镜观察丹参药物血清对ICAM-1表达及胞内Ca2+的影响。


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METHOD Human umbilicus vein endothelial cell and lipopolysaccharide were used to establish apoptosis cell models,and flow cytometer and confocal microscope were used to observe the effect of the medicated serum on ICAM-1 expression and intracellular Ca2+. 方法取人脐静脉内皮细胞(HUVEC),用脂多糖造成凋亡细胞模型,通过流式细胞仪、激光共聚焦显微镜观察丹参药物血清对ICAM-1表达及胞内Ca2+的影响。
METHOD OF MEASURE GLYCOSYLATED SERUM PROTEIN WITH α-D-GLUCOSE PENTAACETATE(α-D-GPA) AS A STANDARD REFERENCE MATERIAL AND STUDY OF REACTIVE MECHANISM 以α-D-GPA为标准参照物测定糖化血清蛋白的方法及其反应机理初探
METHOD:after cultured 72 hour in vitro,cytokines (IL-2、IL-4、IL-10、IFN-γ、TNF-α) in supernatant secreted by PBMCs were detected by ELISA. HCV RNA in serum were assayed by fluorescent quantitation PCR. 方法:体外培养慢性HCV感染患者PBMCs72小时后,ELISA检测培养上清细胞因子(IL-2、IL-4、IL-10、IFN-γ、TNF-α)含量,荧光定量PCR检测患者血清HCV RNA含量。
METHODS 110 batches of FFP, human immunoglobulin and human serum albumin were detected for anti-HCV, HCV RNA, anti-HIV (1+2) 、HIV-1RNA by ELISA, western blot (WB) and PCR. 方法用ELISA、WB和PCR对110批FFP、人血丙种球蛋白、人血白蛋白检测了抗HCV、HCVRNA、抗HIV(1+2)、HIV-1RNA。
METHODS the pharmacokinetics of EPC and HEPC sterically stabilized liposomes (EPC-SSL, and HEPC-SSL) were studied by HPLC. 方法用高效液相色谱法研究EPC和HEPC长循环阿霉素脂质体在大鼠体内的药物动力学。
METHODS: Reverse transcription polymerase chain reaction (RT-PCR) technique was used to detect the expression of β-G mRNA in 10 cases of normal liver tissue and 38 cases of different differentiation tissues of hepatocellular carcinoma. 方法采用逆转录-聚合酶链反应(RT-PCR)方法,对10例正常肝脏及38例不同分化程度肝癌组织中β-G mRNA的表达进行对比研究。
 
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