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Methods suspected chancre specimens which were collected in Hengyang were screened by TP polA PCR. Positive specimens were performed to amplify ARP gene and TPR gene of TP. The TPR gene products were digested by restriction endonuclease.
中文意思:
方法收集衡阳地区疑似硬下疳的标本例,经TP polA PCR筛选后,阳性者用PCR扩增各标本中TP的ARP和TPR基因,酶切TPR基因扩增产物,分析各TP菌株ARP基因长度和TPR基因的限制性片段长度多态性(RFLP),进行分型。


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Methods 8 cases of specimens of lhtty heartall COllie from autopsy. 研究方法 8例脂肪心皆来自尸体解剖。
Methods 8 cases of follicular thyroid carcinomas with infiltration of the peplos were observed by introperative frozen sections and routine paraffin embedded sections,and compared with 0 cases of thyroid adenoma and 80 cases of nodular goiter. 方法对8例有包膜浸润的甲状腺滤泡性癌的术中冰冻切片和术后石蜡切片,与0例甲状腺腺瘤和80例结节性甲状腺肿的术中冰冻切片和术后石蜡切片标本进行了观察比较。 结果滤泡性癌不同于甲状腺腺瘤和结节性甲状腺肿的组织学表现: 滤泡大小不等,以小滤泡为主,其上皮有轻度以上的异型性;
Methods 8 healthy Wistar rats were distributed to groups randomly,using stereotactic guidance to group A:infuse saline 0μl into the right caudate; 方法大鼠8只,随机分为 组:A组:生理盐水组,利用立体定向仪向大鼠右侧尾状核注入生理盐水0μl;
Methods 8 patients of biliary surgery with epidural block were randomly divided into two groups:demerol group(group D, n =) and control group(group C, n =) The demerol (0.~mg/kg) was administered in vein 0 min before skin incision in group D. 方法择期硬膜外阻滞下胆系手术病人 8例 ,随机分为哌口替啶组 (D组 )与对照组 (C组 )各 例。 D组于手术切开皮肤前 0分钟静脉给予哌口替淀 0 ~ mg/kg。
Methods 9 children with HBsAg carrier and 0 healthy children were blooded without eating in the morning. The serum levels of IL-0,IL-,IFN-γ of 9 HBsAg carrier were measured by specific-ELISA. 方法9例HBsAg携带患儿和 0例健康儿童均于清晨空腹采血,以双抗夹心ELISA法检测血清IL-0、IL-和IFN-γ的含量。
Methods suspected chancre specimens which were collected in Hengyang were screened by TP polA PCR. Positive specimens were performed to amplify ARP gene and TPR gene of TP. The TPR gene products were digested by restriction endonuclease. 方法收集衡阳地区疑似硬下疳的标本例,经TP polA PCR筛选后,阳性者用PCR扩增各标本中TP的ARP和TPR基因,酶切TPR基因扩增产物,分析各TP菌株ARP基因长度和TPR基因的限制性片段长度多态性(RFLP),进行分型。
Methods 77 cases with liver disease including acute hepatitis ( 7),chronic hepatitis and cirrhosis (7) were checked by gastro scope and HP test. 方法对77例急慢性肝炎(急性肝炎7例,慢性肝炎 例)和肝硬化(7例)患者进行胃镜检查及HP检测。
Methods patients with HCC were separated into two groups randomly, the observing group(n= ) with devascularization of half of liver, and the control group(n= 0) with Pringle’s devasularization. 方法对例原发性肝癌(HCC)患者随机分为观察组进行半肝血供阻断术(n= )和对照组Pringle法阻断血流切肝术(n= 0);
Methods 77 patients with CHF were divided to three groups according to NYHA and 0 hearty subjects were taken as control,sFas andβ_-MG were measured. 方法将心功能不全患者77例依据NYHA心功能分级分为 组,同时选择健康人 0例作为正常对照组,分别检测研究对象的sFas和β-MG水平。
Methods 8 nasopyaryngeal carcinoma patients,7 nasitis patients and 0 healthy control were selected. EBVCA-IgA and EBNA-IgA in serum were determined to evaluated the diagnostic value of the combined determination of the two indexes. 方法:分别采用免疫酶法和ELISA间接法检测0例正常人,7例鼻炎患者和8例鼻咽癌患者血清中EB病毒壳抗原IgA(VCA IgA)和EB病毒核抗原IgA(VNA-IgA),评价两项指标联合检测对鼻咽癌的诊断价值。
Methods 8 patients above 0 years old with palpitation, stuffy chest, hard breath, lower ST-T, and low and flat T wave were analyzed by taking 0 mg of propranolol orally and observing ECG changes at 0, 0, 90 and 0 min. 方法分析8例0岁以上伴有心悸、胸闷、气短症状,静息心电图有ST段水平或下斜型压低,T波低平的患者,通过口服心得安药物0 mg,分别在 0、0、90、0 min描记心电图观察其变化。
 
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