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MethodsNucleotides within glycosylation motif were changed to a human mutant CD9 gene(from K-H to K-K) by overlap extension PCR. The mutant CD9 gene was inserted into a eukaryotic-expressing vector pALTER to form a recombinant plasmid.
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方法以正常的CD9基因全长cDNA序列为模板,应用重叠延伸PCR法产生突变使CD9糖基化基序K-H突变为K-K,产生突变CD9基因,将其克隆于pALTER质粒中,构建出重组真核表达载体。 |
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MethodsReaction of L-α-Val with ZnAc_·H_O in methanol yields the clubbed crystal, which is identified as Zn(Val)_·H_O by chemical and elemental analyses.
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果 化学分析与元素分析结果表明,其可能组成为Zn(Val)·HO。 用X 射线衍射法测定了它的晶体结构,属正交晶系,P空间群,晶胞参数为:a=0 7 (0)nm; |
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Method：Nineteen embalmed pelves and articulated proximal / of the femurswere harvested for this study.
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髋臼后壁骨折测量完整髋臼、后壁 / 、/ 、 / 骨折时髋臼与股骨头之间的接触特性---负重、负重面积、平均应力、峰值应力和应力的分布情况。 |
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Methof:The mm defects of rat ischium nerve were treated with allografted nerves kept at - 0℃ for 0 days.
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方法：用— 0℃保存0天的同种异体神经桥接大鼠坐骨神经mm缺损； |
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Methord:We extend sclera valve area( mm× mm),imprest infiltration. insure ideal postoperation eye pressure and prevent scar.
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方法 :扩大巩膜瓣设计面积 (mm× mm) ,加强滤过 ,确保理想的术后眼压 ,防止瘢痕形成。 |
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Methylbenzoethyl)sydnone and -( -hydroxyl- -phenylpropyl)sydnone undergo Mannich reaction with aromatic amines and give the corresponding Mannich bases.
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(甲基苯甲酰基乙基 )斯德酮和 ( 羟基 苯基丙基 )斯德酮可以与芳香胺发生Mannich反应 ,得到斯德酮与芳香胺的Mannich碱 |
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Metoprolol ( .0× 0--. × 0-M ) and bupranolol ( . × 0--. × 0-M ) could significantly increase the total QO of pig's heart mitochondria in an hour's experiment using Warburg's apparatus, and , -dinitrophenol .×0-M) also increase it.
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美心安(.0×0~(-)M～.×0~(-)M)和氯甲苯心安(.×0~(-)M～.×0~(-)M)能显著增加猪心肌线粒体小时总耗氧量,,—二硝基苯酚(.×0~(-)M)有同样的作用。 |
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Metro System Planning in Moscow
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莫斯科地铁系统规划 |
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Meximum rates of fertilization reach morethan 98%, with an average from 0% to 80% using the dropper method'.
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而滴管法授精，受精率最高可达到98％，平均值也在0％－80％之间。 |
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Mg powder and B powder with a ratio of .0 ∶ were well mixed and pressed into pellets. Samples were sintered for h at 0,700,70,800,80 and 900℃,respectively,in a flow of high purity argon gas.
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Mg粉、B粉按.0 ∶的比例混合、压制成型后,在流通高纯氩气的条件下分别于0、700、70、800、80和900℃烧结h,制备出MgB块材。 |
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Mg-Protoporphyrin Ⅸ (MP) , a tetrapyrrole intermediate in the plastid act as a plastid signal.
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质体中进行的四吡咯代谢的中间产物镁—原卟啉Ⅸ(Mg-ProtoporphyrinⅨ,MP)就是这样一类质体信号。 |
