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Methods:Enzyme linked immunosorbent assay(ELISA)was used to determine serum SHBG levels in 22 newborns with Gestational diabetes mellitus and 21 with normal pregnant women.
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方法采用酶联免疫吸附双抗体夹心法(ELISA)测定22例GDM患者和21例正常妊娠妇女新生儿血清中SHBG的浓度,用放免电化学发光法检测血清中的胰岛素(INS)浓度。 |
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Methods:The effect of dopamine(DA) on apoptotic PC12 cell were examined by fluorescence microscopy using the DNA end-labeling methods.
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方法：用脱氧核糖核酸（DNA）标记法，借助荧光显微镜观察多巴胺对PC12细胞凋亡的影响。 |
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Methods:The genome DNA was distilled from Tetrandra Root,and differentiate it by RAPD(the random amplification polymorphism DNA).
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方法:从独活药材中提取基因组DNA,运用RAPD(随机扩增多态性DNA)技术对其进行鉴别。 |
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Methods:The levels of IL-1α、IL-1β、IL-17、OPG in 50 RA patients and 32 nomal controls(NC) were detected by using enzyme linked immunosorbent assays(ELISA).
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方法:采用酶联免疫吸附试验检测50例RA患者及32例正常对照组血清中IL-1α、IL-1β、IL-17、OPG和CRP的水平。 |
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Methods:The mouse 11beta-HSD1siRNA plasmids were transfected into 3T3-L1 cells for the examination of the effect of targeted 11beta-HSD1 inhibition on differentiation of 3T3-L1 cells.
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方法:构建11β-HSD1-SiRNA表达质粒pGCsilencerTMH1/TetO1-11β-HSD1并稳定转染3T3-L1细胞,采用油红染色观察脂滴堆积情况,采用Westernblot方法检测11β-HSD1在正常3T3-L1细胞分化过程中的表达; |
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Methods:The result of electrophoresis,purity and enzyme digesting of genome DNA of three kind of Pinaceae were compared.
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方法:分别比较了用传统的CTAB法、SDS法以及3种改良的CTAB法提取的上述3种松科植物叶片基因组DNA的电泳、纯度和酶切效果。 |
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Methods:The-455nt polymorphisms in the βfibrinogen gene were detected by polymerase chair reaction amplication of fragments of the DNA.
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方法β纤维蛋白原基因-455nt多态性分析:(1)碘化钾法提取人基因组DNA; |
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Methods:We constructed a partial double-strand DNA with T7 promoter as DNA template and synthesized small hairpinRNA in-vitro using T7 RNA polymerase.
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方法：根据端粒酶hTERT基因1573—1591位的核酸序列，构建带T7启动子的部分双链DNA模板，用T7RNA聚合酶体外合成短链shRNA。 |
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MethodsITP children,102 cases,were divided into two groups: HBV-ITP group(25 cases) and non-HBV-ITP group(77). All the children were given high dose intravenous immunoglobulin.
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方法将102例ITP病儿,分为两组,其中HBV-ITP组25例,非HBV-ITP组77例,病儿均采用大剂量丙种球蛋白、常规治疗量泼尼松、小剂量长春新碱等治疗,比较两组病儿的临床特征、治疗反应、病程特点等。 |
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MethodsSerum MCP-1,TNFαand IL-6 were detected by radioimmunoassay and enzyme linked immunosorbent assay(ELISA)4 weeks before and after oral administration of pravastatin in 25 patients with UA.
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方法采用放射免疫法及酶联免疫吸附法(ELISA)分别检测了25例UA患者服用普伐他汀前后血清MCP-1、TNFα及IL-6水平。 |
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MethodsSome reaction condition was optimized by amplifying technology and method of PCR based on isolating genomic DNA from D.
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方法在提取纯化美花石斛基因组DNA的基础上,利用PCR扩增技术和方法,对Mg2+,dNTP,模板DNA,引物,Taq聚合酶等反应条件进行优化。 |
