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Total RNA was prepared from immature Beijing wild soybean seeds by SDS-phenol method, and the total mRNA was purified by oligo(dT) cellulose affinity chromatography. The ratio of mRNA to total RNA was about 5.3%.
中文意思:
以SDS—苯酚法从北京野生大豆未成熟种子中制备总核糖核酸(RNA),经Oligo(dT)—纤维素柱亲和层析获得总信使核糖核酸(mRNA),约占总RNA的5.3%。


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Total RNA was extracted from the human Molt-4 cells as template according to the instructions and then human CD81 gene was was amplified by RT-PCR and PCR. The PCR products was determined by Agarose gel electrophoresis. 根据已公布的人CDS IcDNA序列(核酸库中的编号:NM-004356),设计一对引物,然后取传代后的人Molt一4细胞,按照产品操作说明书从人Molt一4细胞提取总RNA,运用RT一PcR及PcR方法扩增出人CD81基因,PCR产物经l%琼脂糖凝胶电泳鉴定大小正确。
Total RNA was prepared from immature Beijing wild soybean seeds by SDS-phenol method, and the total mRNA was purified by oligo(dT) cellulose affinity chromatography. The ratio of mRNA to total RNA was about 5.3%. 以SDS—苯酚法从北京野生大豆未成熟种子中制备总核糖核酸(RNA),经Oligo(dT)—纤维素柱亲和层析获得总信使核糖核酸(mRNA),约占总RNA的5.3%。
Totally 128 differential expressed genes might be related to azoospermia were found by the cDNA microarray hybridization, among them 56 were up regulated genes and 72 were down regulated. 2、共获得128个可能与无精子症相关的差异表达基因,其中56个基因表达上调,72个基因表达下调,cDNA微矩阵杂交结果经原位杂交证实。
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Toxicology Studies on Gynura divaricata in the South China Wild Vegetable 华南野生蔬菜白仔菜的毒理学研究
Toxicology and safety of extract of compound Chinese medicinal herbs 复方中草药提取物的毒理学和安全性研究
 
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