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Methods Gelatin, collagen and RGD peptide Arginine Glycine Aspartic acid were physically adsorbed on PS surfaces, XPS was employed to investigate the variation of nitrogen content on the modified surfaces to validate the existence of proteins.
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方法采用物理包被法依靠疏水作用在PS表面架构明胶、胶原和RGD(精氨酸-甘氨酸-天冬氨酸)多肽的生物活性层。 通过光电子能谱(XPS)分析修饰表面的元素含量变化,N元素含量显著提高,说明蛋白分子在表面存在。 |
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Methods HBV DNA S gene were amplified by polymerase chain reaction(PCR) in sera from 36 children with HBsAg positive after immunized with hepatitis B vaccine. The PCR products were sequenced.
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方法收集乙肝疫苗免疫后HBsAg阳性的儿童血清标本36份,利用聚合酶链反应(PCR)扩增HBV S基因片段,对PCR产物进行脱氧核糖核酸(DNA)序列测定。 |
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Methods Levels of Lp(a) were measured with enzyme-linked immunosorbent assay(ELISA) and genetic phenotypes of Apo(a) were mapped using SDS-polyacrylamide gel electrophoresis(SDS-PAGE) and Western blotting analysis.
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方法应用酶联免疫吸附试验测量血清脂蛋白(a)浓度应用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和Western blotting技术显示载脂蛋白遗传表型。 |
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Methods Neural stem cells both from prime culture(P0) and twice-passaged culture(P2) were tested by enzyme immunoassay for proliferation and fluorescent activated cell sorting for apoptosis by Annexin V-FITC and propidium iodide.
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方法原代培养后(P0)和经过两次传代后(P2)的神经干细胞用BrdU细胞增生检测试剂盒检测增生能力; 用Annexin V-FITC凋亡试剂盒检测早期凋亡率; |
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Methods Plasma levels of oxLDL and sICAM-1 were measured by enzyme-linked immunosorbent assay(ELISA) in 50 women with PE, including 25 mild preeclampsia(MPE) and 25 severe preeclampsia(SPE), and 25 normal pregnant women.
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方法采用酶联免疫吸附法(enzyme-linked immunosorbent as-say,ELISA),检测50例PE患者[PE组,其中子癎前期轻度(mild preeclampsea,MPE)及子癎前期重度(severe preeclampsia,SPE)各25例]及25例同期正常孕妇(对照组)血浆中oxLDL和sICAM-1的水平变化。 |
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Methods S-P immunohistochemical method was used to determine the expression of VEGF-C and nm23-H1 gene protein in 121 human colorectal cancer cases after surgical treatment.
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方法应用免疫组化S-P法对121例大肠癌手术根治标本进行VEGF-C和nm23-H1基因蛋白测定。 |
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Methods Serum TGFβ1 and TIMP-1 were detected by en- zyme-linked immunosorbent assay and serum HA,LN,PⅢNP were detected by radioimmunoassay in 40 CHB patients and 12 healthy persons. Liver biopsies were performed on the 40 patients.
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方法采用酶联免疫吸附法 (ELISA)检测40例慢性乙型肝炎患者和12名健康体检者血清TGFβ1、TIMP-1水平,RIA检测血清HA,LN、Ⅲ型前胶原氨基端肽(P．Ⅲ NP)水平,40例患者全部进行肝活组织检查,分析TGFβ1、TIMP-1与肝组织纤维化程度分期和炎症活动度分级的关系以及五项指标之间的相关性。 |
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Methods Serum TNF-α and IL-6 were determined by enzyme-linked immunosorbent assay in normal subjects(control group),UA patients treated with and without rosiglitazone.
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方法采用酶联免疫吸附法(ELISA法)测定正常对照组、UA患者罗格列酮干预组和未干预组血清中IL-6、TNF-α水平的变化。 |
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Methods Serum levels of VEGF, VEGF C and uPAR were measured with quantitative enzyme-linked immunosorbent assay in 91 patients with OSCC and in 50 healthy individuals as control group.
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方法用酶联免疫吸附分析法检测91例OSCC患者和50例健康人血清中VEGF、VEGF C和uPAR的水平。 |
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Methods Serum sFas and sFasL were assayed by Enzyme-linked immunosorbent assays(ELISA) in 50 patients with gastric cancer, 36 cases with benign gastric diseases and 32 cases of normal subjects.
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方法:采用酶联免疫吸附法(ELISA)检测50例胃癌患者,36例胃良性疾病和32例正常健康者血清中sFas、sFasL的水平,并比较胃癌组中不同TNM分期的变化。 |
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Methods Test the activity of CD69 and level of IL-2 by cell flow cytometer after colon cancer operation in 33 cases.
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方法应用流式细胞仪检测33例结肠癌患者手术前后外周血中T淋巴细胞CD69的表达百分率及IL-2的表达水平,并与对照组进行比较分析。 |
