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Methods: Cholesterol(50.00 mg/L) was used on human umbilical endothelial cell line,ECV304,48 h later,cell genomic DNA methylation level was detected by HPLC.
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方法:体外培养人脐静脉血管内皮细胞ECV304,以50.00 mg/L的胆固醇作用于ECV304 48 h,提取细胞基因组DNA,用高效液相色谱法(HPLC)检测基因组DNA甲基化水平。 |
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Methods: Classified 39 liver recipients according to treatment protocol (basic treatment, added HGF treatment, adbed RNA treatment) as control group, HGF group and RNA group.
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方法:将病人分为Control组、HGF组、RNA组,分别采用基础治疗、基础治疗+肝细胞生长因子、基础治疗+核糖核酸3种治疗方法。 |
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Methods: Serum levels of TNF-α were measu red by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) in 15 patients with psoriasis arthropathica before and after treatment with MTX.
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方法:应用双抗体夹心酶联免疫吸附法(ELISA),检测15例关节病型银屑病患者经MTX治疗前后外周血清中TNF-α的水平。 |
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Methods: The experiment was performed in 6 adult rabbits with the method of retrograde axonal transportation of horseradish peroxidase (HRP), by which HRP was smeared on the proximal cut end of the left ilioinguinal nerves.
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方法:成年家兔6只,采用HRP逆行追踪方法,在左侧髂腹股沟神经近侧断端涂抹HRP。 |
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Methods: The levels of IL-12 were measured by enzyme linked immunosorbent assay(ELISA) in 64 patients with viral hepatitis and 20 healthy adults subject as controls.
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方法:双抗体夹心酶联免疫吸附法(ELISA)检测64例病毒性肝炎患者及20例正常对照血清IL-12水平。 |
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Methods: The liver function and renal function in 268 cases of hemorrhagic fever with renal syndrome were tested and compared with 30 cases of healthy control group.
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方法:对268例HFRS患者的肝功能和肾功能进行检测,并与30例健康对照组进行比较。 |
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Methods: The quantitative study was made in 47 samples of different ovarian tumor cell DNA content.
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方法 :对 47例不同性质的卵巢肿瘤细胞脱氧核糖核酸 (deoxyribonucleicacid ,DNA)含量进行定量研究。 |
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Methods: The specific IgM antibodies of EV,HSV-I,HSV-II,EBV,ADV and FluV were determined by enzyme-linked immunosorbent assay(ELISA) in the cerebrospinal fluid(CSF) in 92 viral encephalitis and meningitis cases.
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方法:应用酶联免疫吸附(ELISA)方法对92例临床诊断为病毒性脑炎、脑膜炎患儿的脑脊液进行肠道病毒(EV)、单纯疱疹病毒(HSV)I型和II型、EB病毒(EBV)、腺病毒(ADV)及流感病毒(FLuV)特异性IgM抗体检测。 |
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Methods:20 pieces adult corpses(40 sides,male 12 ,female 8)were applied and deep iliac circumflex artery、iliohypogastric nerve、ilioinguinal nerve、lateral femoral cutaneous nerve and their relationships were observed.
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方法 :应用成年尸体2 0具 (40侧 ,男尸 12具、女尸 8具 ) ,观测旋髂深动脉、髂腹下神经、髂腹股沟神经、股外侧皮神经及它们之间的位置关系。 |
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Methods:Apply RT-PCR technique to detect the mRNA expression of Th1/Th2 types cytokines & transcription factor T-bet in PBMC of the patients with alopecia areata stimulated by with PHA.
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方法:用逆转录—聚合酶链反应(RT-PCR)分别检测8例轻型斑秃、12例重型斑秃(肝肾不足型)患者及10例正常人外周血单一核细胞(PBMC)经PHA刺激后Th1型细胞因子(IFN-γ、IL-12)、Th2型细胞因子IL-10及转录因子T-betmRNA的表达水平。 |
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Methods:Both the experience of nursing care carried out with 4Fr single lumen of tube made in USA,and problems occurred in 44 cases of PICC patients undergoing hematopoietic stem cells transplantation were summarized.
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[方法]采用美国生产的4Fr单腔导管,针对44例造血干细胞移植病人应用PICC出现的问题实施的护理经验进行总结。 |
