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Advances of Clinical Pharmacology and Chemotherapy of TAXOL
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紫杉醇的临床药理学和化疗新进展 |
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Advances of DNA extraction methods
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DNA提取方法进展 |
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Advances of Molecular Biology of Fowlpox Virus Genome
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鸡痘病毒分子生物学研究进展 |
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Advances of Research on Molecular Biology of Beet Necrotic Yellow Vein Virus
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甜菜坏死黄脉病毒分子生物学的研究进展 |
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Advances of Research on Molecular Biology of Hepatitis C Virus
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丙型肝炎病毒分子生物学研究进展 |
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Advances of Researches on Triterpene Constituents and Pharmacology of Ganoderma lucidum
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灵芝三萜类成分与药理学研究进展 |
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Advantages of designing experiment teaching in medical microbiology
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浅谈设计性实验在医学微生物学教学中的优势 |
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After 10 h cultivation at 30°C and 5 h induction cultivation at 42°C, the final cell density reached 60 OD600, rhTNFaDK2 was more than 50% of the total amount of protein in E. coli, near 2 g pure rhTNFαDK2 per liter broth was obtained.
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在5L在NBS-Bioflo3000型自动控制发酵罐中采取加速补料的补料分批培养,重组大肠杆菌YK537/pSB-TK经10h30°C培养和5h42°C诱导培养,最终密度达到60OD600,rhTNFα-DK2的表达量占菌体总蛋白的50%以上,每升发酵液纯化可得到近2g的rhTNFα-DK2。 |
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After 20 hours, H_2O_2 was added to final concentration 1%, then culture to 24 hours. In this condition, the biomass was 5.26g/100ml, SOD activity was 2662u/g fresh cell, SOD production was 1.40×10~4u/100ml, which was 99% high than original strain.
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在此条件下,细胞生物量为5.26g/100ml,SOD含量为2662u/g湿菌体,SOD产量为14002u/100ml,产量较出发菌提高99%。 |
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After 3 days 2.5μg/mL of arabinoside cytarabine(Ara-C) was added to the culture for 24h to inhibit the outgrowth of glial cells,mechanocytes and nerve stem cells,etc.
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应用2.5μg/mL阿糖胞苷(Ara-C)对培养3 d的神经元进行24 h干预,去除神经胶质细胞、成纤维细胞、神经干细胞等杂质细胞,以提高神经元产率; |
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After cut down by restriction enzyme,the dhaT gene with own ribosome binding site was inserted into recombining plasmid pMD19-T Simple/gldABC in tandem. Thereafter,the gene of gldABC and dhaT was subcloned into expression vector pET28a(+).
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将限制性内切酶处理后的含有自身核糖体结合位点的dhaT基因插入到质粒pMD19-T Simple/gldABC中gldABC基因的下游,形成重组克隆质粒pMD19-T Simple/gldABC-dhaT,并进一步将gldABC与dhaT串联基因亚克隆到表达载体pET28a(+)上。 |