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3. DNA was extracted from peripheral blood leukocytes.
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3．采集血液样本并提取白细胞和基因组脱氧核糖核酸（DNA）。 |
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3. MLC in the Pca tissues increases along with the decreased cancer cell differentiation degree, associates with Pca TNM clinical staging and with or without lymphatic metastases, and these indicate MLC in Pca reflects the tumor biology behavior.
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(3)Pca组织中MLC随癌细胞分化程度的降低而增加,与Pca临床分期、有无淋巴转移有明显相关性,表明MLC在Pca中反映肿瘤的生物学行为。 |
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3. Under optimum inducement condition (37℃, 0.3 mmol/L IPTG, 4 h), the content of recombinant protein could reach to 19% of total cell protein tested by Gene Genius Bio Imaging System.
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3.最佳诱导条件为:0.3 mmol/L IPTG 37℃诱导4 h,表达量约占菌体总蛋白的19%。 |
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3.The transcription of MC148 gene inserted into Ad-MC148 was confirmed by RT-PCR and protein SDS-PAGE .
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经测定病毒滴度高达3．56X 10＼PFU／ml卜三、经RT－PCR和病毒转染细胞上清蛋白提纯，证明腺病毒重组体Ad－MC 48中有MC148基因并且可转录及翻译表达。 |
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449 stool specimens of outpatients with acute diarrhea of two hospitals in Wuhan were examined between October 1985-January 1986. RNA analysis method involving polyacrylamide gel electrophoresis was used.
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1985年10月至1986年1月,在武汉市两所医院肠道门诊,收集了449例急性腹泻病人的粪便标本,用聚丙烯酰胺凝胶电泳方法进行核酸分析,结果发现75例婴儿轮状病毒RNA阳性,检出率16.70％。 |
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4℃ refrigerator preserve hematopoietic stem cells of bone marrow and peripheral blood
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4℃冰箱保存骨髓及外周血造血干细胞 |
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5-~3H uridine or DL-(4,5-~3H)leucine are injected to study the synthestic functions of RNA and protein, respectively.
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注射[5-~3H]尿嘧啶核苷或D. L(4,5)~3H亮氨酸分别研究核酸或蛋白的合成功能。 |
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5. Analysis test for Nucleic acid revealed that bacteriophage XY-1 was a 6000 bp ,single-stranded RNA bacteriophage.
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5.核酸分析试验显示,噬菌体XY-1为6000 bp的单链RNA噬菌体。 |
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5.5. The cell was incubated at 280C with 5.0% seed culture on ashaker operated at l7Orlmin, L-Tyr was added until 0.5 g L at 1 5hand the cell was collected after 21 h, the enzyme specific activity ashigh as 19.0 mU ? mg~?
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以5％接种量接种，于170r/min，28℃培养15h后添加L-酪氨酸至终浓度0.5g/L，继续培养至21h收集菌体，获得最大酶比活力达19.0mU/mg。 |
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5.its transforming activity. Thefrequency of transformation is 2.1×10~6CFU/μg DNA.
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5.pCB_2具有生物活性,其转化频率为2.1×10~6CFU/μgDNA。 |
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6.The comparison of GPIF immunobiologic activity with the similar drugs.
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6、GPIF与同类药品免疫生物活性比较研究 |
