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| Methods A set of universal primers was designed based on the conservative regions in bacterial 16S ribosomal RNA genes. 7 control bacterial strains,human DNA,HBV-DNA and 37 samples of ascites of patients with cirrhosis were amplified with PCR. |
中文意思：
方法在细菌16S rRNA基因保守区设计一对通用引物,对7种对照菌株、人基因组DNA、HBV-DNA、37份肝硬化患者腹水进行PCR扩增。 |
以下为相关句子列表(点击查看，可以看对应的解释）：
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Methods 216 patients with liver failure were treated with an artificial liver support system in addition to the routine medicinal therapy. 214 patients treated only with routine medicinal therapy as control.
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方法使用人工肝支持系统联合常规治疗方法治疗肝功能衰竭216例,并与214例常规治疗对照。 |
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Methods 5 cases of lymphoblastic NK/T cell lymphomas were collected ,4 cases from lymph nodes and 1 case from nasal cavity, the nature of neoplastic cells was confirmed by immunohistochemistry.
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方法 收集 5例母细胞性NK/T细胞淋巴瘤 ,1例原发于鼻腔 ,4例原发于淋巴结。 |
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Methods 84 STD clinic patients' genitourinary tract secretions were collected for Mg DNA detection by polymerase chain reaction (PCR).
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方法采集84例性病门诊患者泌尿生殖道分泌物,用聚合酶链反应(PCR)技术检测生殖支原体脱氧核糖核酸(MgDNA)。 |
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Methods 87 patients aged from 11 month to 13 years were randomly divided into a treatment group (57 cases) and a control group (30 cases). The treatment group was administrated with Hunan immunoglobulin injection. <2y 300mg/d, 3-6y 600mg/d.
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方法87例11个月～13岁的本科住院患儿随机分为治疗组:57例,对照组:30例,治疗组应用国产人血丙种球蛋白注射液,<2岁300mg/d,3～6岁600mg/d,>7岁600～1200mg/d,肌肉注射,1～2次/d,疗程5～7天。 |
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Methods A proband and his 5 family members with T53→C in intron 1 and C59→A in intron 4 mutations of ApoM gene expression and their lipids were detected by polymerase chain reaction(PCR) direct DNA sequencing in a Han nationality Chinese family.
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方法应用聚合酶链反应DNA直接测序法,检测了1个携带ApoM基因1号内含子T53→C、4号内含子C59→A突变的先证者及其5名家系成员这2个位点的基因型及血脂情况。 |
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Methods A set of universal primers was designed based on the conservative regions in bacterial 16S ribosomal RNA genes. 7 control bacterial strains,human DNA,HBV-DNA and 37 samples of ascites of patients with cirrhosis were amplified with PCR.
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方法在细菌16S rRNA基因保守区设计一对通用引物,对7种对照菌株、人基因组DNA、HBV-DNA、37份肝硬化患者腹水进行PCR扩增。 |
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Methods Amplify Rpf E gene from the genomic DNA of M.tuberculosis H37Rv by PCR,insert into expression vector pET32a(+) and transform to E.
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方法采用PCR方法从结核分枝杆菌H37Rv基因组DNA中扩增出RpfE基因,双内切酶消化后,与同样酶消化的pET32a(+)载体连接,转化大肠杆菌Top10。 |
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Methods Analyze and compare the most successful models,cell automaton and car-following model,and point out their advantage and shortcomings.
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方法对现有比较成功的微观模型、元胞自动机模型和跟驰模型进行分析、比较,指出其不足和优点。 |
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Methods BALB/c mice were subcutaneously injected with 0.8mg of the synthesized peptide to produce the experimental autoimmune myocarditis (EAM) models.
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方法:人工合成含心肌肌球蛋白抗原重链表位长度为30个氨基酸残基的多肽0.8mg,纯化多肽皮下免疫遗传易感BALB/c小鼠,建立自身免疫性心肌炎模型,检测TNF-α和cTnI病变期间的改变。 |
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Methods Base on MIC(morphology,immunology and cytogenetics),nested reverse transiption-polymerase chain reaction(RT-PCR) was performed to detect TEL-AML1 fusion gene.
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方法在MIC分型(形态学、免疫学、细胞遗传学)基础上,采用巢式逆转录-聚合酶链反应(RT-PCR)检测ALL患儿90例TEL-AML1融合基因。 |
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Methods CD117~+ hemopoietic stem cells (HSCs) were isolated and purified from the bone marrow of healthy C57 mice by magnetic affinity cell sorting.
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方法取健康C57小鼠骨髓,通过MACS系统分离、纯化CD117~+造血干细胞; |
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