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Objective: To study the expressions and clinical significances of p16INK4A and p14ARF proteins in squamous carcinoma of the cervix(SCC) and cervical intraepithelial neoplasia(CIN).
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方法:采用免疫组织化学S-P法对95例宫颈鳞癌(SCC)标本、80例宫颈上皮内瘤变(CIN)标本和45例正常宫颈标本进行p16INK4A和p14ARF蛋白的检测。 |
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Methods: The effects of oxidants and reducing agents on maxi-conductance calcium-activated potassium channel in cultured rat trigeminal ganglion neurons by using whole-cell patch-clamp technique.
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方法:采用全细胞膜片钳电生理方法,记录氧化还原药物对三叉神经节细胞大电导钙激活钾通道(BKCa)的影响。 |
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Methods: The China National Knowledge Infrastructure (CNKI) was reviewed, ANOVA and cluster analysis were conducted with the sixty selected articles about patients with different diseases on SCL-90 factors.
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方法:查阅中国期刊数据网,对选取的60篇文章中有关不同疾病患者SCL-90的测量结果进行变异数分析和聚类分析。 |
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Objective: To probe into prep-cultivating time s effect on phage expansion experiment of tubercle bacillus.
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方法:对166例临床诊断为肺结核患者的标本,经液化处理后均进行过夜组(预培养24h)及不过夜组(室温放置6h后)分支杆菌噬菌体生物扩增试验,试验方法按结核分支杆菌噬菌体裂解试剂盒(FAST PlaqueTBTM)操作手册操作,对照分析二者的阳性率和污染率。 |
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Objective:To study the possibility of replacement of nasal bone fracture under nasal endoscope.
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方法:对33例闭合性鼻骨骨折患者,在仰卧位下行鼻内镜检查、直视下行鼻骨塌陷处的局部浸润麻醉、鼻骨复位。 |
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Methods usingquestionnaires to investigate the epidemic situation of flu like cases;collecting nasopharynx mopsto examine flu viruses.
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方法:设计调查表格,调查类流感疫情;采集类流感病例鼻咽拭子标本,利用M DCK细胞传代技术分离流感病毒。 |
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Platelet aggregation and thrombgenesis are detected.
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方法:实时监测犬脑血流量、脑血管阻力、心率、平均动脉血压,检测大鼠全血血小板聚集,检测大鼠动-静脉旁路血栓形成。 |
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The mRNA and protein expression of DDC were examined by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting methods, respectively.
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方法:体外合成多巴脱羧酶的小干扰RNA(siRNA-DDC),转染人胃癌细胞系BGC823,逆转录酶链式反应(RT-PCR)和免疫印迹法(Western blot)方法检测DDC基因和蛋白表达的变化,体外侵袭实验检测转染后细胞侵袭能力的变化。 |
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The positive clones were screened by agarose electrophoresis,and the inserted sequence in the recombinants was detected by DNA sequencing.
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方法:以mU 6pro为载体,化学合成shRNA表达模板,并定向克隆到载体的U 6启动子下游,琼脂糖凝胶电泳法筛选阳性克隆,基因测序法检测插入模板序列的正确性。 |
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Its protoplasts were mutated by UV-irradiation and then regenerated by two cycles of screening.
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方法:以酵母菌株YS5作为出发菌株,将酶解破壁后获得的原生质体进行紫外诱变,通过初筛和复筛进行选育。 |
