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The enzyme activity reached 5.47U/mg crude protein when K. pneumoniae F-1 was induced for 4h by 0.5mmol/L IPTG.
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IPTG诱导表达研究表明,在IPTG浓度为0.5mmol/L时,诱导4h后甲酸脱氢酶表达明显; 发酵过程中甲酸脱氢酶比酶活达到5.47U/mg; |
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The enzyme digestion results showed that the six DNA samples could be digested by EcoRⅠ,and were suitable for further molecular biological experiments.
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酶切实验结果表明,6种海洋微藻的基因组DNA都能被EcoRⅠ酶切,酶切图谱呈弥散状,满足分子水平操作的要求,可直接应用于进一步的分子生物学实验。 |
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The enzyme extraction obtained with sterile water at 28 ℃ for 3h was used for the activity determination.
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30℃固体培养70h,发酵后用无菌水(pH5.0)28℃浸提3h测定酶活性。 |
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The enzyme linked immunosorbent assay(ELISA) was used to measure the levels of tumor necrosis factorα(TNF-α),interleukin-1β(IL-1β),monocyte chemoattractant protein-1(MCP-1) and IL-6 in the serum,lung and renal cortex tissue homogenate in various groups.
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用酶联免疫吸附法(ELISA)测定血清、肺和肾组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、单核细胞趋化蛋白-1(MCP-1)及IL-6水平。 |
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The establishment of a method for the detection of the erythrocyte chemokine recepter and its clinical application
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红细胞趋化因子受体测定方法的建立及临床初步应用 |
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The establishment of animal models of recurrent aphthous ulcer with immunology
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免疫方法建立复发性口腔溃疡动物模型 |
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The establishment of the gene expression profile and cloning the genes associated with differentiation inducing of human glioma cells.*
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人脑胶质瘤细胞诱导分化基因谱的建立及相关基因的克隆 |
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The evaluation of radionuclide liver scanning for the diagnosis of 110 cases with primary hepatic carcinoma
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肝扫描对原发性肝癌诊断的价值(附110例病例分析) |
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The experimental data from the following conditions: pressure: 70MPa, cell mass: water volume=l:10 suspended in deionized water with 2 cycles.
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从而选择采用高压匀浆法批式循环,经2次破碎,操作压力为70MPa、菌体悬浮液浓度为:菌体质量:水体积=1:10。 |
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The experimental study of muscle stem cells in vitro
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骨骼肌干细胞的实验研究 |
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The explore of the clinical effect for treatment of 9 SLE cases by autologous perpheral blood stem cells transplantation
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自体外周血造血干细胞移植治疗系统性红斑狼疮9例临床疗效探讨 |
