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However, overexpression of WNK1 in HEK293 cells exerts no detectable effect on the activity of known substrates which indicates that WNK kinases belong to a novel signal pathway.
中文意思:
高表达WNK激酶家族的一个成员——WNK1在HEK293细胞中,没有检测到已知的作用底物生物活性的变化,说明WNK激酶属于一个新的信号传导通路。


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However both genomic DNA and nuclear matrix DNA showed a 940 bp positive fragment with primersⅠ except NM DNA100. The sequence of 940 bp was identical to the DNA sequence of EGFR gene. 940 bp PCR(引物Ⅰ)产物出现在基因组DNA和除NMDNA100外其他核基质DNA中。 940bp序列与EGFR基因DNA序列完全一致。
However, overexpression of WNK1 in HEK293 cells exerts no detectable effect on the activity of known substrates which indicates that WNK kinases belong to a novel signal pathway. 高表达WNK激酶家族的一个成员——WNK1在HEK293细胞中,没有检测到已知的作用底物生物活性的变化,说明WNK激酶属于一个新的信号传导通路。
However, transfection technique of marrow mesenchymal stem cells existed many problems. 但骨髓间充质干细胞的转染技术还存在很多问题。
However,HIV-1 proviral DNA could be amplified in passage 1 of SF33,IIIB,89.6 and YN19 and passage 2 of 89.6 in Macaque's PBMs. 提取盲传各代细胞脱氧核糖核酸(DNA)进行PCR扩增,结果显示,SF33、ⅢB、YN19、89.6感染猴细胞的第一代和89.6感染猴细胞的第二代,均可以检测到HIV1前病毒核酸。
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