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The positive clones were screened by agarose electrophoresis,and the inserted sequence in the recombinants was detected by DNA sequencing.
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方法:以mU 6pro为载体,化学合成shRNA表达模板,并定向克隆到载体的U 6启动子下游,琼脂糖凝胶电泳法筛选阳性克隆,基因测序法检测插入模板序列的正确性。 |
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Its protoplasts were mutated by UV-irradiation and then regenerated by two cycles of screening.
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方法:以酵母菌株YS5作为出发菌株,将酶解破壁后获得的原生质体进行紫外诱变,通过初筛和复筛进行选育。 |
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Objective:To synthesize taxinine NN-3 and taxinine NN-14 with biological activity against multidrug resistant (MDR) tumor cells.
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方法:以紫杉宁为出发原料,通过选择性水解9,10位的乙酰氧基,制成原乙酸酯后再水解。 |
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Methods:the contents of total saponins in wild and cultivated Radix Caulis Acanthopanacis Senticosi leaves was studied by UV-spectrophotometry with total saponins present in the extract as the primary screening index.
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方法:以总皂苷为指标,用比色法对野生和栽培刺五加叶中皂苷的含量进行测定。 |
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Method: Immunohistochemical staining method was used to study the expression of PCNA and nm23 in 47 cases of brain gliomas and 12 cases of normal brain tissues.
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方法:应用免疫组织化学SP法检测47例脑胶质瘤和12例正常脑组织中PCNA和nm23的表达。 |
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The change of α-actin filament was examined by immunocytochemistry.
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方法POP患者(POP组)20例及无POP的直肠癌患者(对照组)30例行盆底肌活检,光镜及电子显微镜观察盆底肌形态,免疫组织化学检测肌细胞内α-肌动蛋白的表达。 |
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Chrysophanol and rhein showed a good linear relationship in a range of 0.
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方法采用薄层鉴别法鉴别大黄、骨碎补;高效液相色谱法测定大黄素和大黄酚的含量。 |
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Methods The expression of mesothelin and CA125 were examed in local tissues of 30 cases of endometrial carcinoma and 136 lymph nodes by immunohistochemistry(sp).
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方法采用免疫组织化学法检测30例子宫内膜癌患者的原发灶组织30份及136枚淋巴结中的MSLN及CA125的表达。 |
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[Methods] Streptavidin-peroxidase of immunohistochemistry was used to detect the expressions of Survivin and b-FGF and microvessel density in 60 cases of esophageal carcinomat tissures and 30 cases of pericarcinomatous tissues.
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方法采用免疫组织化学法检测60例食管癌和30例食管炎组织中Survivin和b-FGF的表达。 |
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Objective To evalute the tracheotmy of replacement of nasal bone fracture under nasal endoscope.
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方法对117例闭合性鼻骨骨折患者行鼻内镜下检查及鼻骨复位,117例患者均可观察到鼻骨骨折部位及15例伴有鼻中隔骨折,局部麻醉下复位108例,全身麻醉下复位9例。 |
