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After cut down by restriction enzyme,the dhaT gene with own ribosome binding site was inserted into recombining plasmid pMD19-T Simple/gldABC in tandem. Thereafter,the gene of gldABC and dhaT was subcloned into expression vector pET28a(+).
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将限制性内切酶处理后的含有自身核糖体结合位点的dhaT基因插入到质粒pMD19-T Simple/gldABC中gldABC基因的下游,形成重组克隆质粒pMD19-T Simple/gldABC-dhaT,并进一步将gldABC与dhaT串联基因亚克隆到表达载体pET28a(+)上。 |
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After diapause the RNA/DNA ratio and the protein content arise to 7.0 and 133 pig/rag respectively.
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滞育结束后RNA/DNA比值上升为7.04,蛋白含量为133微克/毫克脂肪体。 成虫繁殖期与滞育期不同阶段脂肪体核酸、蛋白量的差异反映卵黄蛋白合成水平。 |
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After injecting mifepristone, Thl-type cytokines were high remarkably comparing with the control experiment while the Th2-type cytokines changing little.
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米非司酮促流产后，Th1型细胞因子的表达明显升高，与对照组相比差异极显著，Th2型细胞因子含量仅在用药12h后有显著下降，但很快升高到正常水平； |
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After the Symphyso-collection (278 genes) of the 300 genes wasgot, cluster analysis was done by Cluster software and TreeView.
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对这 300 个基因取并集(共 278 个基因),用 Cluster 软件及 TreeView 完成系统聚类分析。 |
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After the cultivation for 26h at 40℃; in a 100L fermentor, a spore concentration of 1.07×1010 CFU/mL was obtained with a spore percentage of greater than 93% and a maximum live cell concentration of 1.14×1010CFU/mL.
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当在100L发酵罐上40℃培养26h时，得到的菌体浓度为1.14×10~(10)CFU／mL，芽孢浓度为1.07×10~(10)CFU／mL，芽孢形成率为93％。 |
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After the floating neurosphere was formed in primary culture, some neural stem cells were used for suspension culture whereas part of cells was cultured adhesively to the flask precovered by 0.001% polyornithine and 0.2% gelatin.
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原代培养出现大量悬浮神经干细胞球后,一部分细胞继续悬浮培养,另一部分细胞种入用0.001%多聚鸟氨酸和0.2%明胶包被的培养瓶中贴壁培养。 |
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Aim To introduce a high throughput screen model for PARP-1 inhibitors.
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目的建立体外聚腺苷二磷酸核糖聚合酶-1[Poly(ADP-ribose)polymerase-1,PARP-1]抑制剂的高通量筛选模型,筛选潜在的PARP-1抑制剂。 |
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Aim To observe the effects of Polysaccharides nucleic acid fraction of BCG(BCG-PSN) on the expression of thymus and activation-regulated chemokine protein and mRNA in a murine model of allergic asthma.
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目的观察卡介菌多糖核酸(BCG-PSN)对小鼠哮喘胸腺活化调节趋化因子(thymus and activation regulated chemo-kine,TARC)及mRNA表达的影响。 |
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Aim: To investigate the killing effect of activated PBLs stimulated by CD28 and CD80 MoAB on the human colon carcer cell line when combined with gastrin receptor antagonist in vitro.
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目的:探讨联合应用胃泌素拮抗剂和CD28/CD80单克隆抗体共刺激活化的健康人外周血T淋巴细胞(PBLs)在体外对结肠癌细胞株HT-29的杀伤作用。 |
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Aim: To understand the possible effect of arsenic trioxide (As2O3) on acute lpophocyt-ic leukemia cell line.
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目的：了解三氧化二砷（As2O3）对急性淋巴细胞性白血病细胞系的可能作用。 |
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Aims: In this study human colonic cancer cell line HT-29 stimulated by cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, interferon (IFN)-γ and LPS to appraise their effects on the expression of TLR4, MD-2 and responsiveness to LPS.
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目的:探讨以细胞因子肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、干扰素(IFN)-γ和LPS刺激人结肠癌细胞系HT-29后,TLR4和MD-2的表达及其对LPS反应性的变化。 |
