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After the floating neurosphere was formed in primary culture, some neural stem cells were used for suspension culture whereas part of cells was cultured adhesively to the flask precovered by 0.001% polyornithine and 0.2% gelatin. |
中文意思: 原代培养出现大量悬浮神经干细胞球后,一部分细胞继续悬浮培养,另一部分细胞种入用0.001%多聚鸟氨酸和0.2%明胶包被的培养瓶中贴壁培养。 |
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