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After the floating neurosphere was formed in primary culture, some neural stem cells were used for suspension culture whereas part of cells was cultured adhesively to the flask precovered by 0.001% polyornithine and 0.2% gelatin.
中文意思:
原代培养出现大量悬浮神经干细胞球后,一部分细胞继续悬浮培养,另一部分细胞种入用0.001%多聚鸟氨酸和0.2%明胶包被的培养瓶中贴壁培养。


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1 After cut down by restriction enzyme,the dhaT gene with own ribosome binding site was inserted into recombining plasmid pMD19-T Simple/gldABC in tandem. Thereafter,the gene of gldABC and dhaT was subcloned into expression vector pET28a(+). 已记 看解释
2 After diapause the RNA/DNA ratio and the protein content arise to 7.0 and 133 pig/rag respectively. 已记 看解释
3 After injecting mifepristone, Thl-type cytokines were high remarkably comparing with the control experiment while the Th2-type cytokines changing little. 已记 看解释
4 After the Symphyso-collection (278 genes) of the 300 genes wasgot, cluster analysis was done by Cluster software and TreeView. 已记 看解释
5 After the cultivation for 26h at 40℃; in a 100L fermentor, a spore concentration of 1.07×1010 CFU/mL was obtained with a spore percentage of greater than 93% and a maximum live cell concentration of 1.14×1010CFU/mL. 已记 看解释
6 After the floating neurosphere was formed in primary culture, some neural stem cells were used for suspension culture whereas part of cells was cultured adhesively to the flask precovered by 0.001% polyornithine and 0.2% gelatin. 已记 看解释
7 Aim To introduce a high throughput screen model for PARP-1 inhibitors. 已记 看解释
8 Aim To observe the effects of Polysaccharides nucleic acid fraction of BCG(BCG-PSN) on the expression of thymus and activation-regulated chemokine protein and mRNA in a murine model of allergic asthma. 已记 看解释
9 Aim: To investigate the killing effect of activated PBLs stimulated by CD28 and CD80 MoAB on the human colon carcer cell line when combined with gastrin receptor antagonist in vitro. 已记 看解释
10 Aim: To understand the possible effect of arsenic trioxide (As2O3) on acute lpophocyt-ic leukemia cell line. 已记 看解释
11 Aims: In this study human colonic cancer cell line HT-29 stimulated by cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, interferon (IFN)-γ and LPS to appraise their effects on the expression of TLR4, MD-2 and responsiveness to LPS. 已记 看解释
 
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