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Methods The heparinaseⅡ gene was amplified with PCR from the genomic DNA of Flavobacterium heparinum, and it was linked into expression plasmid pET-28a after sequencing, then the recombined plasmid was expressed in E.
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方法通过PCR从黄杆菌基因组DNA中扩增出黄杆菌HepⅡ的基因,经验证后插入到表达质粒pET-28a上构建表达载体,重组质粒转化E. |
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Methods The level of Th1 and Th2 cytokines in serum were detected by flow fluorenscence immunmicrobeads assay (FFIA) from 44 patients with breast cancer and 47 cases of patients with breast benign Tumour.
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方法用流式荧光免疫微球分析技术(Flow Fluorenscence Immunmicrobeads Assay,FFIA)检测乳腺良、恶性肿瘤患者血清Th1/Th2细胞因子水平。 |
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Methods The samples of serum and BALF were obtained from 47 patients with untreated primary lung cancer and 18 patients with benign pulmonary diseases. The levels of endostatin were analyzed by enzyme-linked immunosorbent assay(ELISA).
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方法采用酶联免疫吸附法(En-zyme-linked immunosorbent assay,ELISA)检测初诊肺癌47例及肺良性病变18例患者外周血清及BALF中Endostatin的表达水平。 |
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Methods The serum anti-β_2GPI and anti-cardiolipin antibodies(ACA) were measured by enzyme-linked immunosorbent assay(ELISA) in 56 patients with SLE and 40 healthy controls.
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方法采用酶联免疫吸附试验(ELISA)半定量测定56例SLE患者及40名健康对照者血清抗β2GPI和抗心磷脂抗体(ACA)阳性率。 |
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Methods The serum concentrations of AOPP and AGE were measured by using spectrophotometer and enzyme linked immunosorbent assay in 29 patients with NSTEMI/UA and 26 patients with STEMI (ACS group) and 30 healthy subjects (control group).
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方法选择ACS患者共55例,其中非ST段抬高ACS(NSTEMI/UA)组29例,ST段抬高ACS(STEMI)组26例,正常对照组30例作为研究对象,采用分光光度计法检测所有入选者的血清AOPP浓度,抗戚蓝蛋白晚期糖基化终末产物抗体竞争酶联免疫吸附测定法测定血清AGE水平,并记录4周内所有患者的病情转归情况。 |
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Methods The serum levels of MPO were determined by enzyme-linked immunosorbent assay(ELISA) in 67 patients diagnosed as coronary artery atherosclerosis by angiography and 20 cases which angiography was normal as control group.
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方法应用酶联免疫吸附试验(ELISA)测定67例经冠状动脉造影证实的冠心病患者和20例造影阴性对照者的MPO水平,冠心病患者按造影结果分为3组。 |
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Methods The serum levels of TNF-α and sTNFRI were determined in 32 healthy subjects and 32 T2DM patients by enzyme-linked immunosorbent assay(ELISA) and ESR was determined by Westergren method.
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方法严格选取32例T2DM患者及32名正常人为研究对象,采用酶联免疫吸附试验(ELISA)分别测定血清TNF-α和sTNFRI水平,采用魏氏法测定ESR。 |
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Methods The serum samples from 75 patients who suffered from the small cell lung cancer were examined by the enzyme-linked immunosorbent assay(ELISA) for SCC,NSE and CYFRA21-1.The relationship among them was analysed by statistics method.
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方法用酶联免疫吸附试验(ELISA)测定75例小细胞肺癌患者的血清SCC、NSE和CYFRA21-1,并计算三者与肿瘤预后的相关性。 |
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Methods The study summarizes virus types,clinical types,characteristics of symptoms,damage to liver,and post-recover damage of 103 dual-infection from 1723 in-patients received in our department in past 10 years.
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方法回顾性分析本科近10年间1723例病毒性肝炎住院病人中103例双重感染的病毒分类、临床分型、症状特点、肝功能损害及预后等。 |
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Methods Two hundred and thirty cases of chronic liver disease were chosen,their liver function,blood sugar,blood insulin and leptin were detected,insulin resistance(IR) was estimated by homeostasis model assessment(HOMA).
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方法对230例慢性肝病患者进行肝功能、血糖、胰岛素及瘦素水平检测,稳态模型估算胰岛素抵抗指数(HOMA-IR)。 |
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Methods Using the DNA endlabeling method the effect of dopamine (DA) on rat apoptotic PC12 cells was examined by flow cytometry and fluorescence microscopy.
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方法用脱氧核糖核酸（DNA）标记法，借助流式细胞仪和荧光显微镜观察多巴胺（DA）对大鼠嗜铬细胞瘤（PC12）细胞凋亡的影响。 |
