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Methods Amino acid sequence of LcrV on secondary structure, LcrV flexibility and hydrophilicity and antigenic index were analyzed using DNAStar Protean softeware and EMBOSS net platform to predict the B cell epitopes.
中文意思:
方法采用EMBOSS网络平台以及DNAStar Protean软件分析LcrV蛋白的二级结构、可塑性、亲水性和抗原性指数等参数,进行B细胞表位的预测和分析。


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Methods AML - ETO genomic fusion sequences were characterized by LD - PCR/LDI - PCR combined with sequence, then the patients specific primers were designed for the detection of AML - ETO fusion gene of the patients' Guthrie cards by PCr; 实验方法用LD-PCR或LDI-PCR联合序列分析确定t(8;)断裂区基因组DNA序列,根据所得序列设计病人特异性引物,用PCR方法检测患者Guthrie cards AML-ETO融合基因;
Methods According to the 0 years' baseline data of AIDS of Xiamen City, the radices of the population at high risk for AIDS were estimated and the epidemic status of AIDS was evaluated by Workbook in Xiamen City. 方法依据厦门市0余年来积累的艾滋病监测及调查的基线数据,运用Work-book法,估计厦门市艾滋病高危人群基数,评估艾滋病疫情。
Methods Admission records of 9c hildren with ovarium cysts operated from Jua. 998to Dec.00,in cludi ngcases treated by routine open operation and8cases treated by laparoscop e were retrospectively analyzed,and was compared. 方法对比998年月至00年月 9例小儿卵巢囊肿手术治疗效果及预后,其中例行传统手术治疗,8例行腹腔镜手术。
Methods After ligating the splenic artery at pancreatic body or tail, the splenic injury was sutured preserving the arteriae gastricae breves and arteria gastroepiploica sinistra. 方法 剪开胃结肠韧带,在胰体尾后上方找到脾动脉后结扎,保留胃短动脉和胃网膜左动脉对脾脏的血供,并缝合脾裂口。
Methods After the relative surgical treatment of different cases of knee arthrosis pyogenic infection with kneescope,according to the principle of the dialectical method. The honeysuckle- amboyna liquid was used to lavage the location? 方法对例不同类型的膝关节感染病人在使用膝关节镜辅助下,进行相应外科处理后,按照辩证论治原则,使用银黄灌洗液进行局部灌洗。
Methods Amino acid sequence of LcrV on secondary structure, LcrV flexibility and hydrophilicity and antigenic index were analyzed using DNAStar Protean softeware and EMBOSS net platform to predict the B cell epitopes. 方法采用EMBOSS网络平台以及DNAStar Protean软件分析LcrV蛋白的二级结构、可塑性、亲水性和抗原性指数等参数,进行B细胞表位的预测和分析。
Methods Antisense oligodeoxynucleotide (ASODN) technique was employed to inhibit endogenous noggin expression and immunohistochemistry was used to detect the expressions of Nestin and GFAP in the hippocampus of adult rats. 方法反义寡核苷酸技术封闭内源性Noggin基因的表达,免疫组化法检测成年大鼠海马内Nestin与GFAP的表达。
Methods Applying the groin flaps to repair the degloved thumbs in 9 cases,the size of the flaps ranged from 8cmⅹ7cm~cmⅹ8cm,the donor site was sutured directly. 方法应用髂腹股沟皮瓣修复拇指皮肤脱套伤9例,切取皮瓣面积8cm×7cm~cm×8cm,供皮区直接缝合。
Methods Blood sample select standard aerobic bottle for use or detest oxygen bottle increase fungus train,train appearance go on after measuring with VITEK- instrument and determine with Back/Alert0 full-automatic blood card analyses determine. 方法血液标本采用标准需氧瓶或厌氧瓶增菌培养,用Back/Alert0血培养仪进行检测后,用VITECK- 全自动微生物分析仪器及鉴定卡分析。
Methods By using Family Environment Scale(FES-CV) and Simplified Coping Style Questionnaire(SCSQ), freshen from medical college were investigated. 方法运用家庭环境量表(FES-CV)和简易应对方式问卷(SCSQ)对医科大学新生名进行调查。
Methods CD8 gene was cut out from plasmid pCDM8 by restriction endonuclease Xho Ⅰ. It was inserted into eukaryotic expression vector pCDNA and identified by restriction enzyme BamH Ⅰ. 方法 用限制性内切酶XhoⅠ酶切质粒pCDM8得到CD8基因 ,并将其插入pCDNA ,用BamHⅠ酶切鉴定。
 
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