英汉双语例句-生物医药大词典

After 10 h cultivation at 30°C and 5 h induction cultivation at 42°C, the final cell density reached 60 OD600, rhTNFaDK2 was more than 50% of the total amount of protein in E. coli, near 2 g pure rhTNFαDK2 per liter broth was obtained. 

中文意思：
在5L在NBS－Bioflo3000型自动控制发酵罐中采取加速补料的补料分批培养，重组大肠杆菌YK537／pSB－TK经10h30°C培养和5h42°C诱导培养，最终密度达到60OD600，rhTNFα－DK2的表达量占菌体总蛋白的50％以上，每升发酵液纯化可得到近2g的rhTNFα－DK2。

以下为相关句子列表(点击查看，可以看对应的解释）：

	Advances of Molecular Biology of Fowlpox Virus Genome			鸡痘病毒分子生物学研究进展
	Advances of Research on Molecular Biology of Beet Necrotic Yellow Vein Virus			甜菜坏死黄脉病毒分子生物学的研究进展
	Advances of Research on Molecular Biology of Hepatitis C Virus			丙型肝炎病毒分子生物学研究进展
	Advances of Researches on Triterpene Constituents and Pharmacology of Ganoderma lucidum			灵芝三萜类成分与药理学研究进展
	Advantages of designing experiment teaching in medical microbiology			浅谈设计性实验在医学微生物学教学中的优势
	After 10 h cultivation at 30°C and 5 h induction cultivation at 42°C, the final cell density reached 60 OD600, rhTNFaDK2 was more than 50% of the total amount of protein in E. coli, near 2 g pure rhTNFαDK2 per liter broth was obtained. 			在5L在NBS－Bioflo3000型自动控制发酵罐中采取加速补料的补料分批培养，重组大肠杆菌YK537／pSB－TK经10h30°C培养和5h42°C诱导培养，最终密度达到60OD600，rhTNFα－DK2的表达量占菌体总蛋白的50％以上，每升发酵液纯化可得到近2g的rhTNFα－DK2。
	After 20 hours, H_2O_2 was added to final concentration 1%, then culture to 24 hours. In this condition, the biomass was 5.26g/100ml, SOD activity was 2662u/g fresh cell, SOD production was 1.40×10~4u/100ml, which was 99% high than original strain.			在此条件下,细胞生物量为5.26g/100ml,SOD含量为2662u/g湿菌体,SOD产量为14002u/100ml,产量较出发菌提高99%。
	After 3 days 2.5μg/mL of arabinoside cytarabine(Ara-C) was added to the culture for 24h to inhibit the outgrowth of glial cells,mechanocytes and nerve stem cells,etc.			应用2.5μg/mL阿糖胞苷(Ara-C)对培养3 d的神经元进行24 h干预,去除神经胶质细胞、成纤维细胞、神经干细胞等杂质细胞,以提高神经元产率;
	After cut down by restriction enzyme,the dhaT gene with own ribosome binding site was inserted into recombining plasmid pMD19-T Simple/gldABC in tandem. Thereafter,the gene of gldABC and dhaT was subcloned into expression vector pET28a(+).			将限制性内切酶处理后的含有自身核糖体结合位点的dhaT基因插入到质粒pMD19-T Simple/gldABC中gldABC基因的下游,形成重组克隆质粒pMD19-T Simple/gldABC-dhaT,并进一步将gldABC与dhaT串联基因亚克隆到表达载体pET28a(+)上。
	After diapause the RNA/DNA ratio and the protein content arise to 7.0 and 133 pig/rag respectively.			滞育结束后RNA/DNA比值上升为7.04,蛋白含量为133微克/毫克脂肪体。成虫繁殖期与滞育期不同阶段脂肪体核酸、蛋白量的差异反映卵黄蛋白合成水平。
	After injecting mifepristone, Thl-type cytokines were high remarkably comparing with the control experiment while the Th2-type cytokines changing little.			米非司酮促流产后，Th1型细胞因子的表达明显升高，与对照组相比差异极显著，Th2型细胞因子含量仅在用药12h后有显著下降，但很快升高到正常水平；