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3.The transcription of MC148 gene inserted into Ad-MC148 was confirmed by RT-PCR and protein SDS-PAGE .
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经测定病毒滴度高达3．56X 10＼PFU／ml卜三、经RT－PCR和病毒转染细胞上清蛋白提纯，证明腺病毒重组体Ad－MC 48中有MC148基因并且可转录及翻译表达。 |
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449 stool specimens of outpatients with acute diarrhea of two hospitals in Wuhan were examined between October 1985-January 1986. RNA analysis method involving polyacrylamide gel electrophoresis was used.
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1985年10月至1986年1月,在武汉市两所医院肠道门诊,收集了449例急性腹泻病人的粪便标本,用聚丙烯酰胺凝胶电泳方法进行核酸分析,结果发现75例婴儿轮状病毒RNA阳性,检出率16.70％。 |
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4℃ refrigerator preserve hematopoietic stem cells of bone marrow and peripheral blood
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4℃冰箱保存骨髓及外周血造血干细胞 |
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5-~3H uridine or DL-(4,5-~3H)leucine are injected to study the synthestic functions of RNA and protein, respectively.
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注射[5-~3H]尿嘧啶核苷或D. L(4,5)~3H亮氨酸分别研究核酸或蛋白的合成功能。 |
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5. Analysis test for Nucleic acid revealed that bacteriophage XY-1 was a 6000 bp ,single-stranded RNA bacteriophage.
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5.核酸分析试验显示,噬菌体XY-1为6000 bp的单链RNA噬菌体。 |
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5.5. The cell was incubated at 280C with 5.0% seed culture on ashaker operated at l7Orlmin, L-Tyr was added until 0.5 g L at 1 5hand the cell was collected after 21 h, the enzyme specific activity ashigh as 19.0 mU ? mg~?
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以5％接种量接种，于170r/min，28℃培养15h后添加L-酪氨酸至终浓度0.5g/L，继续培养至21h收集菌体，获得最大酶比活力达19.0mU/mg。 |
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5.its transforming activity. Thefrequency of transformation is 2.1×10~6CFU/μg DNA.
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5.pCB_2具有生物活性,其转化频率为2.1×10~6CFU/μgDNA。 |
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6.The comparison of GPIF immunobiologic activity with the similar drugs.
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6、GPIF与同类药品免疫生物活性比较研究 |
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66 workers exposed to vibration and 66 controls were investigated using "neurobehavioral core test battery"(NCTB) recommended by WHO.
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本文应用世界卫生组织推荐的神经行为核心测试组合（WHO·NCTB）对66名接振工人及66名对照人员进行了神经行为功能测试。 |
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94.7% patients treated with glucocorticoid in the earlier period,57.9% patients received intravenous immunoglobulin (IVIG) treatment meanwhile.
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94.7%的病例早期给予糖皮质激素治疗,57.9%的病例同时给予丙种球蛋白静滴(IVIG)。 |
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<title>Apoptosis and corneal transplantation immunology
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细胞凋亡与角膜移植免疫 |
