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However,HIV-1 proviral DNA could be amplified in passage 1 of SF33,IIIB,89.6 and YN19 and passage 2 of 89.6 in Macaque's PBMs.
中文意思:
提取盲传各代细胞脱氧核糖核酸(DNA)进行PCR扩增,结果显示,SF33、ⅢB、YN19、89.6感染猴细胞的第一代和89.6感染猴细胞的第二代,均可以检测到HIV1前病毒核酸。


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However both genomic DNA and nuclear matrix DNA showed a 940 bp positive fragment with primersⅠ except NM DNA100. The sequence of 940 bp was identical to the DNA sequence of EGFR gene. 940 bp PCR(引物Ⅰ)产物出现在基因组DNA和除NMDNA100外其他核基质DNA中。 940bp序列与EGFR基因DNA序列完全一致。
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However, transfection technique of marrow mesenchymal stem cells existed many problems. 但骨髓间充质干细胞的转染技术还存在很多问题。
However,HIV-1 proviral DNA could be amplified in passage 1 of SF33,IIIB,89.6 and YN19 and passage 2 of 89.6 in Macaque's PBMs. 提取盲传各代细胞脱氧核糖核酸(DNA)进行PCR扩增,结果显示,SF33、ⅢB、YN19、89.6感染猴细胞的第一代和89.6感染猴细胞的第二代,均可以检测到HIV1前病毒核酸。
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