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Total RNA was extracted from the human Molt-4 cells as template according to the instructions and then human CD81 gene was was amplified by RT-PCR and PCR. The PCR products was determined by Agarose gel electrophoresis.
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根据已公布的人CDS IcDNA序列(核酸库中的编号:NM-004356)，设计一对引物，然后取传代后的人Molt一4细胞，按照产品操作说明书从人Molt一4细胞提取总RNA，运用RT一PcR及PcR方法扩增出人CD81基因，PCR产物经l%琼脂糖凝胶电泳鉴定大小正确。 |
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Total RNA was prepared from immature Beijing wild soybean seeds by SDS-phenol method, and the total mRNA was purified by oligo(dT) cellulose affinity chromatography. The ratio of mRNA to total RNA was about 5.3%.
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以SDS—苯酚法从北京野生大豆未成熟种子中制备总核糖核酸(RNA),经Oligo(dT)—纤维素柱亲和层析获得总信使核糖核酸(mRNA),约占总RNA的5.3％。 |
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Totally 128 differential expressed genes might be related to azoospermia were found by the cDNA microarray hybridization, among them 56 were up regulated genes and 72 were down regulated.
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2、共获得128个可能与无精子症相关的差异表达基因，其中56个基因表达上调，72个基因表达下调，cDNA微矩阵杂交结果经原位杂交证实。 |
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Toxicology Evaluation of Qiangshen Yishou Ye
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强身益寿液的毒理学评价 |
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Toxicology Experimental Study on Gonghuanzhixue Pills
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宫环止血片毒理学实验研究 |
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Toxicology Studies on Gynura divaricata in the South China Wild Vegetable
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华南野生蔬菜白仔菜的毒理学研究 |
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Toxicology and safety of extract of compound Chinese medicinal herbs
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复方中草药提取物的毒理学和安全性研究 |
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Toxicology experiment study of WuDiDan capsule
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无敌丹胶囊毒理学实验研究 |
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Toxicology study on HPV16 and its genes induce cervical cancer in Mouse
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HPV16及其基因诱导小鼠宫颈癌的毒理学研究 |
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Toxicology study on cyanotacterium microcystis and microcystins pollution in freshwater lake
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淡水湖泊微囊藻毒素的污染和毒理学研究 |
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Transcriptional Regulation of Differentiation-associated Genes by Transcription Factor c-Jun/AP-1 in Esophageal Squamous Cell Carcinoma
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转录因子c-Jun/AP-1对食管鳞癌分化相关基因转录调控机理研究 |
