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Methods AML - ETO genomic fusion sequences were characterized by LD - PCR/LDI - PCR combined with sequence, then the patients specific primers were designed for the detection of AML - ETO fusion gene of the patients' Guthrie cards by PCr;
中文意思:
实验方法用LD-PCR或LDI-PCR联合序列分析确定t(8;)断裂区基因组DNA序列,根据所得序列设计病人特异性引物,用PCR方法检测患者Guthrie cards AML-ETO融合基因;


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1 Methods 8 patients above 0 years old with palpitation, stuffy chest, hard breath, lower ST-T, and low and flat T wave were analyzed by taking 0 mg of propranolol orally and observing ECG changes at 0, 0, 90 and 0 min. 已记 看解释
2 Methods A HPLC method was used with DiamonsilTM C 8(0× . mn, μ m) column and a mobile phase of methanol- 0. % phospho ric acid solution (adjusted the pH to .0 with triethylamine)(0 ∶ 0). 已记 看解释
3 Methods A PE-0 catheter with a silicon was used to block the middle cerebral artery(MCA) of rats for hours in cold saline infusion group,and locally infuse the MCA-supplied territory with . ml 0℃ saline for minutes before reperfusion. 已记 看解释
4 Methods A comparison study was made between 0 patients wearing monocles and 0 patients wearing progressive multi-focal spectacles about their degree of phoria taken with Von Graefe test at the start of wearing the spectacles and , months later. 已记 看解释
5 Methods A total of patients(mean age,7 years;age range, -9 years) with serum PSA> ng/ml or positive rectal examination results or abnormal B-ultrasonography findings underwent transperineal sextant biopsy and more cores of the nodules. 已记 看解释
6 Methods AML - ETO genomic fusion sequences were characterized by LD - PCR/LDI - PCR combined with sequence, then the patients specific primers were designed for the detection of AML - ETO fusion gene of the patients' Guthrie cards by PCr; 已记 看解释
7 Methods According to the 0 years' baseline data of AIDS of Xiamen City, the radices of the population at high risk for AIDS were estimated and the epidemic status of AIDS was evaluated by Workbook in Xiamen City. 已记 看解释
8 Methods Admission records of 9c hildren with ovarium cysts operated from Jua. 998to Dec.00,in cludi ngcases treated by routine open operation and8cases treated by laparoscop e were retrospectively analyzed,and was compared. 已记 看解释
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10 Methods After the relative surgical treatment of different cases of knee arthrosis pyogenic infection with kneescope,according to the principle of the dialectical method. The honeysuckle- amboyna liquid was used to lavage the location? 已记 看解释
11 Methods Amino acid sequence of LcrV on secondary structure, LcrV flexibility and hydrophilicity and antigenic index were analyzed using DNAStar Protean softeware and EMBOSS net platform to predict the B cell epitopes. 已记 看解释
 
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