以下为句子列表：
英文: Research on E.coli protease system is the basis of engineering strain improvement In order to reduce their negative effect, much has been done, among which antisense RNA technology is worth further trying.
中文: 为减少蛋白酶对外源蛋白的降解，从不同的角度做了大量工作，其中利用反义RNA技术控制蛋白酶活性是一条值得探索的思路。
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英文: The end cell density OD600 of the engineering strain pNSR-16/BL21(DE3)pLysS was 60-90, and the expression of the recombinant spider silk protein was up to the 11-12% of the total protein by the Do-stat fed-batch culture.
中文: 采用Do-stat补料分批发酵,最终菌体密度OD_(600)可在60-90范围,产物表达量约占总蛋白量的11～12%。
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英文: When the strain was cultivated in a 5 L fermenter,dry cell weight increased to 8.06 g/L from 3.12 g/L. The high-cell density growth of engineering strain has no negative effect on the expression of target protein, and the production of rhAGN was 2.51 g/L.
中文: 利用此合成培养基进行5 L罐的发酵试验,菌体干重由摇瓶下的3.02 g/L提高到8.06 g/L,同时,试验表明,菌体浓度提高后,目的蛋白的表达未受影响,依然稳定在35%左右,rhAGN的表达量达到2.51g/L。
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英文: L-glutamate Production by Engineering Strain with Temperature and Lysozyme Sensitivity
中文: 温度和溶菌酶敏感菌株的L-谷氨酸合成
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英文: Expression of Hemoglobin Gene in the Pichia pastoris Engineering Strain of Producing CBHⅡ
中文: 血红蛋白基因在产CBHⅡ毕赤酵母工程菌中的表达
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