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When the strain was cultivated in a 5 L fermenter,dry cell weight increased to 8.06 g/L from 3.12 g/L. The high-cell density growth of engineering strain has no negative effect on the expression of target protein, and the production of rhAGN was 2.51 g/L.
中文意思:
利用此合成培养基进行5 L罐的发酵试验,菌体干重由摇瓶下的3.02 g/L提高到8.06 g/L,同时,试验表明,菌体浓度提高后,目的蛋白的表达未受影响,依然稳定在35%左右,rhAGN的表达量达到2.51g/L。


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When detecting foot-and-mouth disease virus VPl in distilled RNA, When the quantity of nucleic acid exceed or be equal to 0. 23ng/μl, RT-PCR can detect; 通过部标RT-PCR方法和nested PCR方法的敏感性比较研究,部标RT-PCR方法能检测出样品中病毒核酸量大于或等于0.23ng/μl的被检材料;
When the DO was controlled above 30%, carbon and nitrogen source was feeded at log phase,the dry cell weight and the expression level of reCPI-GST reached 26.2 g/L and 24.8% of the total cell protein, respectively. 在3.7 L台式搅拌发酵罐中,控制溶氧在30%以上,重组菌的对数生长中期流加碳源和氮源,最终菌体光密度A600达到52.4,菌体干重为26.2g/L,目的蛋白的表达量达到24.8%。
When the body is unable to endure the antigens of islets, both MHC-Ⅱand its disposel antigens of β cells would activate Th1 subgroup ofhelper T lymphocytes (Th), and restrain Th2 subgroup, which results inthe unbalance of cytokines. 当机体对胰岛多种抗原成分失去耐受后,主要组织相容性复合物(MHC)-Ⅱ类分子与其处理的β细胞抗原共同激活辅助性 T 淋巴细胞(Th)中的 Th1 亚群,抑制 Th2 亚群,造成 Th1/Th2 型细胞因子的不平衡;
When the bone marrow is preserved in liquid nitrogen, the viability and function of the cells can be sustained much longer. The recoverable rate of the nuclear cells, exclusion of trypon blue, DNA and CFU-C arc 76%, 89%, 77% and 79% respectively. 骨髓贮存于液氮中,其细胞的活力和功能得以较长保存,有核细胞、锥蓝拒染率、脱氧核糖核酸(DNA)和CFU—C的回收率分别为76%、89%、77%和79%.
When the direction of modification is parallel to the basic vector of Si (111) 7×7 surfaces, grooves formed by such a process have atomically straight edges and lateral features as small as one 7×7 unit cell wide. 当这种表面改形的取向与Si(111)7×7表面晶格基矢方向平行时,形成的沟槽将具有愿子级平直的边缘,横向宽度仅为一个7×7晶胞。
When the strain was cultivated in a 5 L fermenter,dry cell weight increased to 8.06 g/L from 3.12 g/L. The high-cell density growth of engineering strain has no negative effect on the expression of target protein, and the production of rhAGN was 2.51 g/L. 利用此合成培养基进行5 L罐的发酵试验,菌体干重由摇瓶下的3.02 g/L提高到8.06 g/L,同时,试验表明,菌体浓度提高后,目的蛋白的表达未受影响,依然稳定在35%左右,rhAGN的表达量达到2.51g/L。
When treated with 100mg/L、250mg/L or 500mg/L Ag+, the cell wall was loosely, uneven, and the widened space between cell wall and membrane was seen clearly. Ag+含量100mg/L、250mg/L、500mg/L处理组作用后,可见到金葡菌细胞壁松散,胞壁与胞膜分离等不同程度的破坏,菌体肿胀,胞浆电子密度降低,直至菌体破裂。
Which is the best dosage of intravenous immunoglobulin for treatment of Kawasaki disease: a comparative investigation on clinical and immunologic effects 优选静脉免疫球蛋白剂量治疗川崎病临床与免疫学效应研究
With further studies and developments of transplantation immunology, eliminating transplant rejection through inducing immune tolerance has become the main goal in organ transplantation. 而随着移植免疫学的进一步研究和发展,诱导免疫耐受已经成为器官移植研究的主要目标。
With the development of Combinatorial Chemistry and high throughput screening technology, the speed of drug discovery has improved dramatically. 随着组合化学和高通量筛选技术的发展,药物研发速度发生了质的飞跃,但其中40%化合物会因代谢性质较差而被淘汰。
With the development of immunology,research found CCR5 and CCR3 could be used as the specific surface makers of Thl and Th2 cells. 近年来,国外免疫学研究发现CCR5/CCR3可以作为Th1细胞/Th2细胞特异性的表面标志。
 
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