以下为句子列表：
英文: (3) Universal DNA polymerase was used in PCR amplification instead of Pfu enzyme;
中文: (3)在PCR反应中用普通的DNA聚合酶代替高保真酶(Pfu);
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英文: On the basis of constructed rolB and pttGA20ox gene plant vector by restriction endonuclease and PCR method with high fidelity DNA polymerase of pfu and pyrobest, the binary vector was constructed with both that two genes.
中文: 在首次利用高保真的pfu与pyrobest DNA聚合酶构建rolB及pttGA20ox单价基因植物表达载体的基础上，构建了由这两个基因组成的双价基因植物表达载体；
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