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The optimal conditions for immobilizing cells were obtained with cross act experiment: quantity of damp cell 4.8g/L, CacL_2 concentration%, SA concentration 5%.
中文意思:
通过正交实验法确定了制备固定化细胞的最优操作条件为:湿菌体量4.8g/L(湿菌体质量/海藻酸钠体积),氯化钙质量浓度为2%,海藻酸钠质量浓度5%。


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The nonspecificresistance and immunology function changes of the mice exposed to CH3Br were studied. The mice were exposed to CH3Br(385?3mg/m3,770?2mg/m3)twice at o,3 days for 2 hours. 实验观察澳甲烷(CH_3Br)染毒小鼠非特异性抵抗力和免疫功能变化,实验小鼠分别用CH_3Br(385±23mg/m~3、770±32mg/m~3)染毒2h,3天后复染1次,染毒后1周分别测定非特异性抵抗力和免疫功能改变。
The number of fracture achieve anatomical reduction, imperfect reduction, unsatisfactory reduction of standard and modified ilioinguinal approach group was 13, 7, 4 , 29, 4 and 2 respectively. 标准和改良髂腹股沟入路组达到解剖复位、复位欠佳和不满意复位的病例数分别为13、7、4例和29、4、2例。
The numbers of CD3+T lymphocyte and CD68+ macrophage increased significantly in the interstitial tissue as compared with that of control group(P<0.05),and infiltration of both cell types was found in the tubules. CD3+T淋巴细胞、CD68+巨噬细胞较对照组明显增多(P<0.05),并浸润到生精小管中。
The objective of this paper is to Study on the therapeutical basis of Rhododendrom micranthum on anti-prostatic hyperplasia action by pharmacology and serum pharmacochemistry methods and to isolate the effective chemical components from the plant; 第一部分照山白抗前列腺增生药理作用研究目的:研究照山白提取液抗前列腺增生作用,确定照山白抗前列腺增生作用的有效部位,并进一步研究其有效部位的抗炎、镇痛、抗前列腺增生作用。
The obtained ethylene receptor genes with two open reading frames,referred to ZjETR1 and ZjERS1,encoded 738 and 632 amino acids,respectively. 在分析已知序列基础上,分别通过3′RACE及对两个基因上游5′端编码区的扩增,得到了两个包含完整开放阅读框(ORF)以及3′端非编码区(3′UTR)的乙烯受体编码基因,即ZjETR1和ZjERS1。 它们分别编码738个和632个氨基酸。
The optimal conditions for immobilizing cells were obtained with cross act experiment: quantity of damp cell 4.8g/L, CacL_2 concentration%, SA concentration 5%. 通过正交实验法确定了制备固定化细胞的最优操作条件为:湿菌体量4.8g/L(湿菌体质量/海藻酸钠体积),氯化钙质量浓度为2%,海藻酸钠质量浓度5%。
The optimum pH range of 6.0—7.0, the optimum temperature of 25℃ and the optimum glucose concentration of 10% were observed for maximum cell concentration in calcium alginate beads in seven days. 实验结果表明在pH6.0~7.0、温度25℃、葡萄糖浓度为10%的条件下,固定化运动发酵单胞菌增殖速度最快,并于7天内达到最大菌体浓度。
The optimum parameters are: enzyme solution pH5.3, enzymatic hydrolysis temperature 40℃, enzyme 10mg/g and time of enzymatic hydrolysis 3 hours. 确定了酶解的最优条件为:酶解液初始pH值5.3,酶解温度为40℃,酶用量为10mg/g,酶解时间为3h。
The optimum reactive pH was 7,an d the recombinant enzyme activity changed little when incubated in the buffer of pH8~10 on 37℃ for 1 h. 重组酶的最适反应pH是7.0,37℃下在pH8~10的缓冲液中保温1h酶活几乎不改变。
The optimum reactive temprature was 75℃,and the re combinant enzyme was more stable on the temperature of 25~55℃. 重组酶反应的最适温度为75℃,酶活稳定的温度范围为25~55℃。
The optimum temperature was 30 ℃,but not stable at temperature above 30 ℃ The optimum pH for the enzyme activity was 7.5.Its activity can be stimulated by K+,Na+,Ca2+ and Mn2+,but strongly inhibited by Hg2+,Zn2+ and Cu2+. 酶反应最适pH为7.5,最适反应温度为30℃,30℃下保温30 min,酶活力基本不变,高于30℃酶活力则迅速下降; K+、Na+、Ca2+、Mn2+对酶活性有促进作用,Hg2+、Zn2+和Cu2+等对酶有抑制作用。
 
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