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Method Neural stem cells were transfected by recombined rAAV2/eGFP and rAAV2/NGF virus after separa tion,culture and characterization,then we detected the expression of GFP and NGF by fluorescence microscopy and immunocytochemistry in vitro.
中文意思:
方法 分离、培养和鉴定神经干细胞后,用重组rAAV2 /eGFP和rAAV2 /NGF病毒转染神经干细胞,用荧光显微镜和免疫细胞化学检测GFP和NGF在体外的表达。


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Membrane Oxidation Lesions of Stored Red Cell and Protecting Effect of Antioxidation Enzymes 贮存红细胞膜氧化损伤与抗氧化酶的保护作用
Meningococcus Group A Immunoglobulin Therapy for Meningococcal Infection A群脑膜炎双球菌免疫球蛋白对脑膜炎双球菌感染的治疗
Mesenchymal Stem Cells Transplantation for Repairing Infarcted Myocardium 骨髓间充质干细胞移植修复梗死心肌的应用基础研究
Method Anti -HCV, HCV RNA and anti-HIV were detected using real time PCR and ELISA. 方法 采用实时定量聚合酶链反应 (PCR)和酶联免疫吸附试验 (ELISA)法等检测了抗 HCV、HCV核糖核酸 (RNA)和抗 HIV。
Method HIV-1 RNA VL of 87 plasma specimens were quantified by method of the NASBA HIV-1 RNA QT System respectively at the collection time of 0,3,6,11,12,13,and 14th month,during which plasma specimens were stored at(-20℃). 方法应用HIV-1核酸序列扩增系统技术(NASBA)对87份-20℃保存的HIV-1抗体阳性血浆样本分组,分别在第0、3、6、11、12、13、14个月进行HIV-1病毒载量检测。
Method Neural stem cells were transfected by recombined rAAV2/eGFP and rAAV2/NGF virus after separa tion,culture and characterization,then we detected the expression of GFP and NGF by fluorescence microscopy and immunocytochemistry in vitro. 方法 分离、培养和鉴定神经干细胞后,用重组rAAV2 /eGFP和rAAV2 /NGF病毒转染神经干细胞,用荧光显微镜和免疫细胞化学检测GFP和NGF在体外的表达。
Method Sixty-one type 1 diabetic mellitus(TIDM)pediatric patients(28 with DKA,33 without DKA)and 30 healthy controls were recruited in this study. Serum IL-18,IL-12,and IFN-Υlevels were measured in both groups by enzyme linked immunosorbent assay(ELISA). 方法采用酶联免疫吸附(ELISA)方法检测61例儿童1型糖尿病(T1DM)患儿(其中28例伴DKA,33例无DKA)和30例正常对照者IL-18、IFN-γ和IL-12的血清水平。
Method The clinical data were collected and the HSV Ⅱ DNA was detected by fluorescence quantitative PCR(FQ PCR)methods for 221 cases with suspected genital HSV Ⅱ infection,and the clinical data of 136 cases with genital HSV Ⅱ infection were analyzed. 方法 对 2 2 1例生殖器部位可能有HSV Ⅱ感染者采集了临床资料 ,并应用荧光探针定量聚合酶链反应技术 (FQ PCR)对其进行了HSV Ⅱ脱氧核糖核酸 (DNA)定量检测 ,并分析了其中 136例HSV Ⅱ阳性患者的临床资料。
Method The effect of dopamine(DA) and glutathione (GSH) on apoptotic PC12  cell were examined by fluorescence microscopy using the DNA endlabeling methods. 方法用脱氧核糖核酸(DNA)标记法,借助荧光显微镜观察多巴胺(DA)和还原型谷胱甘肽(GSH)对PC12细胞凋亡的影响。
Method for determining existent minimum range of fault elements in control circuits for aluminium reduction cell 铝电解槽控制电路中故障元件存在的最小范围的确定方法
Method of Checking Failure of Aluminium Reduction Cell's Bottom 铝电解槽炉底破损的检查方法
 
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