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Isolation and Cultivation of Murine Hematopoietic Stem Cells and Expression of hFIX Mediated by Recombinant Lentiviral Vectors in vitro
中文意思:
小鼠造血干细胞的分离、培养及重组慢病毒载体介导的离体hFⅨ基因表达


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⑤The c-kit positive cells were about 55.3% polygon cells in cell membrane and cytoplasm,whereas fibroblast-like cells with smaller bodies were negative for c-kit. ⑤体积较大的多角形细胞为c-kit阳性,体积较小的成纤维样细胞为阴性细胞,阳性颗粒主要分布在细胞膜和细胞质,细胞核未见表达,阳性细胞比率为55.3%。
【Methods】Reverse transcription polymerase chain reaction(RT-PCR) was used to detect TLR4 mRNA expression,and enzyme-linked immunosorbent assay(ELISA) was used to examine IL-8 secretion. 【方法】选用人结肠癌细胞株HT-29细胞,采用逆转录聚合酶链反应(RT-PCR)检测HT-29细胞的TLR4 mRNA的表达; 酶联免疫吸附法(ELISA)检测HT-29细胞的IL-8分泌。
㎡bserve the effect of GAPSGF on the serum protein components by SDS-PAGE. ③通过SDS-PAGE,观察GAPSGF对HepA瘤血清蛋白组分的影响;
Results:The difference of the ratio of liver function recuperated and the time of HBV-DNA turned negative in two groups had no significant meaning(P>0.05). 结果:两组肝功能复常率、HBV-DNA转阴时间比较,差异无显著性意义(P>0.05);
Effect of Cigarette Smoke on Maturation of DNA in Sperm Nucleus and Function of Sperm Membrane in Rats 香烟烟雾对大鼠精子核DNA成熟度和精子膜功能的影响
Isolation and Cultivation of Murine Hematopoietic Stem Cells and Expression of hFIX Mediated by Recombinant Lentiviral Vectors in vitro 小鼠造血干细胞的分离、培养及重组慢病毒载体介导的离体hFⅨ基因表达
A study on the interaction between dye and bovine serum albumin 染料分子与牛血清蛋白相互作用的研究(英文)
Application of Method to Eliminate the Heteroduplex Caused in the Course of PCR to Microbial Molecular Ecology Reconditional PCR方法在微生物分子生态学研究中的一种应用
OSTEOGENIC POTENTIAL OF FIBROBLASTS WITH RECONSTITUTED TELOMERASE ACTIVITY 重建端粒酶活性的正常人成纤维细胞的成骨潜能研究
Upper Jurassic conchostracan fossils from central Yunnan and its stratigraphic significance 滇中地区上侏罗统叶肢介化石及其地层学意义
Mapping of an Arabidopsis Gene Involved in Microspore Development 一个控制拟南芥小孢子发育基因的定位
 
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