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After the floating neurosphere was formed in primary culture, some neural stem cells were used for suspension culture whereas part of cells was cultured adhesively to the flask precovered by 0.001% polyornithine and 0.2% gelatin.
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原代培养出现大量悬浮神经干细胞球后,一部分细胞继续悬浮培养,另一部分细胞种入用0.001%多聚鸟氨酸和0.2%明胶包被的培养瓶中贴壁培养。 |
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Aim To introduce a high throughput screen model for PARP-1 inhibitors.
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目的建立体外聚腺苷二磷酸核糖聚合酶-1[Poly(ADP-ribose)polymerase-1,PARP-1]抑制剂的高通量筛选模型,筛选潜在的PARP-1抑制剂。 |
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Aim To observe the effects of Polysaccharides nucleic acid fraction of BCG(BCG-PSN) on the expression of thymus and activation-regulated chemokine protein and mRNA in a murine model of allergic asthma.
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目的观察卡介菌多糖核酸(BCG-PSN)对小鼠哮喘胸腺活化调节趋化因子(thymus and activation regulated chemo-kine,TARC)及mRNA表达的影响。 |
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Aim: To investigate the killing effect of activated PBLs stimulated by CD28 and CD80 MoAB on the human colon carcer cell line when combined with gastrin receptor antagonist in vitro.
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目的:探讨联合应用胃泌素拮抗剂和CD28/CD80单克隆抗体共刺激活化的健康人外周血T淋巴细胞(PBLs)在体外对结肠癌细胞株HT-29的杀伤作用。 |
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Aim: To understand the possible effect of arsenic trioxide (As2O3) on acute lpophocyt-ic leukemia cell line.
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目的：了解三氧化二砷（As2O3）对急性淋巴细胞性白血病细胞系的可能作用。 |
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Aims: In this study human colonic cancer cell line HT-29 stimulated by cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, interferon (IFN)-γ and LPS to appraise their effects on the expression of TLR4, MD-2 and responsiveness to LPS.
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目的:探讨以细胞因子肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、干扰素(IFN)-γ和LPS刺激人结肠癌细胞系HT-29后,TLR4和MD-2的表达及其对LPS反应性的变化。 |
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Aims: To investigate the expression of HPV and its effect on the protein expression of related genes in esophageal carcinoma.
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目的:研究HPV在食管癌中的表达,探讨其对相关基因蛋白表达的影响。 |
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All rats were sacrificed by exsanguinations at 1,3 and 6 h of exposure to gas. Lung lobes were harvested for measuring the levels of tumor necrosis factor-α(TNFα),interleukin-6(IL-6) and interleukin-10(IL-10) with enzyme-linked immunosorbent assay.
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酶联免疫吸附法测定吸入气体1、3、6 h后肺肿瘤坏死因子α(TNF-α)、白细胞介素6及10(IL-6、IL-10)含量,光镜下评分比较损伤变化。 |
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All results were taken statistical analysis. Results:Exposure time of standard and modified ilioinguinal approach group was 42.36±4.58 min and 29.64±3.19 min respectively;
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结果:标准和改良髂腹股沟入路组的切口显露时间(x±s)分别为(42.36±4.58)min和(29.64±3.19)min; |
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Allogeneic peripheral blood stem cells transplantation for treating a patient with β thalassemia major
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异基因外周血造血干细胞移植治疗重型β地中海贫血 |
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Allogeneic transplantation of purified haploidentical CD34~+ peripheral blood stem cells with suitable T cell reinfusion in treating Ph+ALL
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外周血CD34~+造血干细胞加适量T细胞输注进行HLA半相合的异体移植 |
