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Methods CD8 gene was cut out from plasmid pCDM8 by restriction endonuclease Xho Ⅰ. It was inserted into eukaryotic expression vector pCDNA and identified by restriction enzyme BamH Ⅰ.
中文意思:
方法 用限制性内切酶XhoⅠ酶切质粒pCDM8得到CD8基因 ,并将其插入pCDNA ,用BamHⅠ酶切鉴定。


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Methods Amino acid sequence of LcrV on secondary structure, LcrV flexibility and hydrophilicity and antigenic index were analyzed using DNAStar Protean softeware and EMBOSS net platform to predict the B cell epitopes. 方法采用EMBOSS网络平台以及DNAStar Protean软件分析LcrV蛋白的二级结构、可塑性、亲水性和抗原性指数等参数,进行B细胞表位的预测和分析。
Methods Antisense oligodeoxynucleotide (ASODN) technique was employed to inhibit endogenous noggin expression and immunohistochemistry was used to detect the expressions of Nestin and GFAP in the hippocampus of adult rats. 方法反义寡核苷酸技术封闭内源性Noggin基因的表达,免疫组化法检测成年大鼠海马内Nestin与GFAP的表达。
Methods Applying the groin flaps to repair the degloved thumbs in 9 cases,the size of the flaps ranged from 8cmⅹ7cm~cmⅹ8cm,the donor site was sutured directly. 方法应用髂腹股沟皮瓣修复拇指皮肤脱套伤9例,切取皮瓣面积8cm×7cm~cm×8cm,供皮区直接缝合。
Methods Blood sample select standard aerobic bottle for use or detest oxygen bottle increase fungus train,train appearance go on after measuring with VITEK- instrument and determine with Back/Alert0 full-automatic blood card analyses determine. 方法血液标本采用标准需氧瓶或厌氧瓶增菌培养,用Back/Alert0血培养仪进行检测后,用VITECK- 全自动微生物分析仪器及鉴定卡分析。
Methods By using Family Environment Scale(FES-CV) and Simplified Coping Style Questionnaire(SCSQ), freshen from medical college were investigated. 方法运用家庭环境量表(FES-CV)和简易应对方式问卷(SCSQ)对医科大学新生名进行调查。
Methods CD8 gene was cut out from plasmid pCDM8 by restriction endonuclease Xho Ⅰ. It was inserted into eukaryotic expression vector pCDNA and identified by restriction enzyme BamH Ⅰ. 方法 用限制性内切酶XhoⅠ酶切质粒pCDM8得到CD8基因 ,并将其插入pCDNA ,用BamHⅠ酶切鉴定。
Methods CFW in megathermal(atmospheric temperature, 9℃±0.℃) and megahumid environment(relative humidity,80%~8%) was made by pistol in 0 dogs. The dogs with CFW were randomly divided averagely into hypothermia and control groups. 方法选杂种犬0只,制作出高温高湿环境下(气温 9℃±0.℃,相对湿度80%~8%)颅脑火器伤的动物模型,随机分为亚低温组和对照组,每组 0只犬。
Methods CT findings of cases of urachal abnormalities proven by surgery and pathology were retrospectively reviewed, the emphasis was focused on its location, shape, size, enhancement-pattern and radiological features in Retzius space. 方法回顾分析 例经手术病理证实的脐尿管病变的CT表现,包括病灶部位、形态、大小、增强以及Retzius间隙影像改变。
Methods Checklist on Quality of Life issued by World Health Organization (WHOQOL-BRIEF) and Social Support Rating (SSRS) were used to conduct a survey in 8 discharged patients suffering from traumatic paraplegia. 方法采用世界卫生组织生存质量测定量表简表(WHOQOL-BRIEF)和社会支持评定量表(SSRS)对8例外伤性截瘫患者在出院后进行追踪调查,并对其生存质量与社会支持关系进行相关性分析。
Methods Chest CT images in cases of pectus excavatum from 00-0 —00-07 in our hospital were retrospectively analysed. 方法对我院00-0 —00-07间经手术治疗的 例患漏斗胸的患儿胸部CT检查进行回顾性的分析。
Methods Clinical symptom and laboratory test were analyzed of 0 patients with Eosinophilic prurigo(A group) and 0 patients with Prurigo simplex(B group). 方法对嗜酸性痒疹(A组) 0例、寻常性痒疹(B组) 0例的临床症状及实验室检查等资料进行对比分析。
 
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