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Methods:Both the experience of nursing care carried out with 4Fr single lumen of tube made in USA,and problems occurred in 44 cases of PICC patients undergoing hematopoietic stem cells transplantation were summarized.
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[方法]采用美国生产的4Fr单腔导管,针对44例造血干细胞移植病人应用PICC出现的问题实施的护理经验进行总结。 |
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Methods:E2Fp was amplified from the genome DNA of HepG-Ⅱ cells, then inserted into the multiple cloning sites of the plasmid pGL3-Basic to generate the pGL3-E2Fp.
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方法:提取HepG-Ⅱ细胞基因组DNA,PCR扩增其转录因子E2F启动子(E2Fp),插入pGL3-Basic多克隆位点,构建成pGL3-E2Fp; |
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Methods:Enzyme linked immunosorbent assay(ELISA)was used to determine serum SHBG levels in 22 newborns with Gestational diabetes mellitus and 21 with normal pregnant women.
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方法采用酶联免疫吸附双抗体夹心法(ELISA)测定22例GDM患者和21例正常妊娠妇女新生儿血清中SHBG的浓度,用放免电化学发光法检测血清中的胰岛素(INS)浓度。 |
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Methods:The effect of dopamine(DA) on apoptotic PC12 cell were examined by fluorescence microscopy using the DNA end-labeling methods.
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方法：用脱氧核糖核酸（DNA）标记法，借助荧光显微镜观察多巴胺对PC12细胞凋亡的影响。 |
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Methods:The genome DNA was distilled from Tetrandra Root,and differentiate it by RAPD(the random amplification polymorphism DNA).
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方法:从独活药材中提取基因组DNA,运用RAPD(随机扩增多态性DNA)技术对其进行鉴别。 |
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Methods:The levels of IL-1α、IL-1β、IL-17、OPG in 50 RA patients and 32 nomal controls(NC) were detected by using enzyme linked immunosorbent assays(ELISA).
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方法:采用酶联免疫吸附试验检测50例RA患者及32例正常对照组血清中IL-1α、IL-1β、IL-17、OPG和CRP的水平。 |
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Methods:The mouse 11beta-HSD1siRNA plasmids were transfected into 3T3-L1 cells for the examination of the effect of targeted 11beta-HSD1 inhibition on differentiation of 3T3-L1 cells.
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方法:构建11β-HSD1-SiRNA表达质粒pGCsilencerTMH1/TetO1-11β-HSD1并稳定转染3T3-L1细胞,采用油红染色观察脂滴堆积情况,采用Westernblot方法检测11β-HSD1在正常3T3-L1细胞分化过程中的表达; |
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Methods:The result of electrophoresis,purity and enzyme digesting of genome DNA of three kind of Pinaceae were compared.
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方法:分别比较了用传统的CTAB法、SDS法以及3种改良的CTAB法提取的上述3种松科植物叶片基因组DNA的电泳、纯度和酶切效果。 |
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Methods:The-455nt polymorphisms in the βfibrinogen gene were detected by polymerase chair reaction amplication of fragments of the DNA.
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方法β纤维蛋白原基因-455nt多态性分析:(1)碘化钾法提取人基因组DNA; |
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Methods:We constructed a partial double-strand DNA with T7 promoter as DNA template and synthesized small hairpinRNA in-vitro using T7 RNA polymerase.
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方法：根据端粒酶hTERT基因1573—1591位的核酸序列，构建带T7启动子的部分双链DNA模板，用T7RNA聚合酶体外合成短链shRNA。 |
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MethodsITP children,102 cases,were divided into two groups: HBV-ITP group(25 cases) and non-HBV-ITP group(77). All the children were given high dose intravenous immunoglobulin.
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方法将102例ITP病儿,分为两组,其中HBV-ITP组25例,非HBV-ITP组77例,病儿均采用大剂量丙种球蛋白、常规治疗量泼尼松、小剂量长春新碱等治疗,比较两组病儿的临床特征、治疗反应、病程特点等。 |
