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Objective To prepare tanshinone ⅡA inclusion complex freeze-dried powder injection and study its characters.
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方法以羟丙基-β-环糊精(HP--βCD)为包合材料,采用冷冻干燥法制备注射用丹参酮ⅡA包合物;用差示扫描量热法、红外光谱法及相溶解度法验证包合物的形成;考察注射用丹参酮ⅡA包合物复溶后的pH值、澄清度、渗透压、与稀释剂的配伍稳定性及溶血性等基本性质。 |
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Methods The RT-PCR for AQP1 mRNA, Western blotting for protein, and immunohistochemistry for distribution in the seminal vesicle and prostate gland on postnatal day 15, day 35 and day 70 were carried out.
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方法应用RT-PCR、免疫印迹和免疫组织化学染色方法,检测出生后15d、35d、70d小鼠精囊腺、前列腺AQP1 mRNA、蛋白表达水平及细胞定位。 |
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Methods The inhibitory rate on cell proliferation of NSCLC in celecoxib group,DDP group,VP16 group,DDP with celecoxib group and VP16 with celecoxib group was detected by MTT assay.
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方法应用噻唑蓝比色法(MTT)检测顺铂(DDP)、足叶乙甙(VP16)单独应用及与塞来昔布联合应用的增殖抑制率,并根据金氏公式计算Q值,评价两药的合用效应。 |
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Methods Whole genome amplification (WGA) was performed for trace DNA using modified IPEP, and the amplified products were quantitated by real-time quantitative PCR as well as genotyped by AmpFLSTR~ Indentifiler~ kit.
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方法用改良IPEP法对痕量样品DNA进行全基因组扩增(WGA),扩增产物用实时荧光定量PCR技术定量、用AmpFLSTR~ Indentifiler~试剂盒作基因型检测。 |
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Methods Acute cutaneous toxic experiment was performed on the skin of healthy rats;the skin irritation test was performed on the skin of rabbit;the cutaneous hypersensitivity test was performed on the skin of guinea pig skin.
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方法用健康大鼠进行急性毒性试验;用家兔进行皮肤刺激性试验;用健康豚鼠进行皮肤过敏试验。 |
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Method Enzyme-linked Immuno-sorbent Assay (ELISA) and western blotting were used to detect the antibody produced by the 95 patients with SARS and test the specificity of virus spike protein antigen.
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方法运用酶联免疫法(ELISA)和蛋白质印迹法(WesternBlotting)检测95例SARS患者体内抗体的产生情况和验证病毒Spike蛋白抗原的特异性。 |
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Liver stage proteins were analyzed by Western-blot.
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方法制备针对疟原虫唾液腺子孢子的免疫血清,通过免疫印迹识别肝细胞内疟原虫裂殖体的相关蛋白,并对识别的蛋白进行肽指纹图谱分析。 |
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Physiological and biochemical index at the stage of bolting Saposhnikovia divaricata(Turcz.) Schischk;
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防风抽薹期生理生化指标分析 |
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Isolation and structure identification of chemical constituents from Saposhnikovia divaricata(Turcz.) Schischk;
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防风化学成分的分离与结构鉴定 |
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Application of RAPD in Fangfeng;
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防风及其近缘种的RAPD分析 |
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TLC identification of Saposhnikovia divaricata and its che mical constituents;
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防风及其主要化学成分的TLC鉴定 |
