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The best condition selected in this study for the protoplast preparation is that: 15 hr for cell age . 0. 01 M for pretreatment concentration of β-mercaptoethanol and about 60 min for enzymolysis time.
中文意思:
经实验确认,原生质体制备最优条件为:取菌龄为15hr的菌体,以0.01mol/L的β—巯基乙醇预处理,蜗牛酶温浴时间为60min.


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The application of SPR technology in the biology- biochemistry analysis and clinic immunology SPR技术在生物、生化分析以及临床免疫学中的应用
The arrival process to each input port is ONOFF (Active and Silence) cell stream with Pareto distribution. 输入端口信元的到达过程是ON-OFF突发流,且ON态以概率p发送信元,ON-OFF长度为Pareto分布的随机变量;
The assay system of the biological activity of lymphotoxin was established using L929 cell as the sensitive target, LT international standard as the positive control and crystal violet staining method to detect viable cell after treated with LT. 为确定经上述步骤纯化后得到的目的蛋自LT△27的生物活性,本研究以L929细胞为靶细胞、淋巴毒素国际标准品为参照,采用结晶紫染色法检测经淋巴毒素处理后存活的细胞,对淋巴毒素生物活性测定的细胞接种浓度、淋巴毒素标准品板上稀释的起始浓度和梯度稀释的倍数、放线菌素D的使用剂量等进行条件实验后,建立了人淋巴毒素生物活性测定方法。
The association of three genes of renin-angiotensin system with diabetic retinopathy 肾素-血管紧张素系统三个基因与糖尿病视网膜病变的相关性
The basic principle of DNA extraction from soybean 大豆DNA提取基本原理的探讨
The best condition selected in this study for the protoplast preparation is that: 15 hr for cell age . 0. 01 M for pretreatment concentration of β-mercaptoethanol and about 60 min for enzymolysis time. 经实验确认,原生质体制备最优条件为:取菌龄为15hr的菌体,以0.01mol/L的β—巯基乙醇预处理,蜗牛酶温浴时间为60min.
The binding reaction of DNA (fsDNA,ctDNA) with victory blue B has been studied. 本文研究了阳离子染料维多利亚蓝 B与脱氧核糖核酸 (fs DNA、ct DNA)的结合反应。
The biolgic activity and clinical significance of calcitonin gene 降钙素基因相关肽的生物活性及其临床意义
The biologic effects of hyperthermia and radiation on gastric cancer cells(SGC-7901) in vitro——Ⅰ. Influence of heating and radiation on cell growth curves, number of colonies and mitosiss of cells. 高温与辐射对人胃癌细胞系(SGC-7901)的生物效应——Ⅰ.对细胞生长曲线、集落形成率、有丝分裂等的影响
The biological activity of IL 1 β reached its peak at 24 h after WBI with 2.0 Gy and it was significantly increased at 24 h after WBI with 0.075 , 0.2, 0.5, 1.0, 2.0 and 4.0 Gy. ( 剂量效应关系结果表明 ,0 .0 75、0 .2、0 .5、1.0、2 .0、4 .0、6.0 Gy照射后 2 4 h,小鼠腹腔巨噬细胞 IL- 1β蛋白生物活性均高于对照组 ,除 6.0 Gy组外 ,其余各组均显著高于对照组
The biological activity of catalytic domains of collagenase 1 and stromelysin 1 showed obvious difference under high pressure: the activity of collagenase 1 increased with elevating pressure, with an apparent activation volume of -18.9 ml/mol; 胶原酶I和基质酶I在高压力下的生物活性变化亦有所不同 :随着压力升高 ,胶原酶I水解底物的活性显著增加 ,1.8kbar时表观活性为常压下的 3倍多 ,由此估算胶原酶I的表观活化体积为 - 18.9ml/mol。
 
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