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AIM: To observe the enhanced green fluorescent protein (EGFP) gene expression and cytotoxicity in human adipose derived adult stem cells (hADSCs) by pEGFP-N1, Ad5-EGFP and rAAV-2/1-EGFP, and investigate the suitable gene-transferred vehicle.
中文意思:
目的:观察脂质体介导质粒pEGFP-N1、重组腺病毒Ad5-EGFP、重组腺相关病毒rAAV-2/1-EGFP基因转染人脂肪来源成体干细胞后增强型绿色荧光蛋白的表达及细胞毒性,探讨适用于脂肪来源的成体干细胞的转基因方法。


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AIM: Feeder layer cells are the necessary condition to prevent the differentiation of embryonic stem cells cultured in vitro. 目的:为保持胚胎干细胞的高度未分化状态,饲养层细胞成为胚胎干细胞体外培养中不可缺少的条件。
AIM: To investigate the differentiation of bone marrow stromal stem cells (BMSCs) into hepatocytes in vitro and the reparation of liver cells after BMSCs transplantation in mice with liver injury. 目的:研究骨髓基质干细胞(BMSCs)在体外向肝细胞的定向分化和体内移植后对小鼠受损肝脏的修复作用.
AIM: To investigate the expression of Wnt-1 in hippocampus after cerebral ischemia/reperfusion (I/R) in rats, and explore the molecular mechanism on regulating the proliferation and differentiation of neural stem cells. 目的:检测wnt-1在大鼠脑缺血再灌注海马组织神经干细胞早期增殖分化中的表达及其变化,探讨影响神经干细胞早期增殖分化的分子调控机制。
AIM: To isolate human epidermal stem cells by type Ⅳ collagen, and explore its effect on the cellular biological characteristics. 目的:评估Ⅳ型胶原黏附法分选表皮干细胞的分选效果及对细胞生物学特性的影响。
AIM: To observe influences of ischemic brain injury on proliferation and transference of endogenous neural stem cells. 目的:观察缺血性脑损伤对内源性神经干细胞增殖、迁移的影响。
AIM: To observe the enhanced green fluorescent protein (EGFP) gene expression and cytotoxicity in human adipose derived adult stem cells (hADSCs) by pEGFP-N1, Ad5-EGFP and rAAV-2/1-EGFP, and investigate the suitable gene-transferred vehicle. 目的:观察脂质体介导质粒pEGFP-N1、重组腺病毒Ad5-EGFP、重组腺相关病毒rAAV-2/1-EGFP基因转染人脂肪来源成体干细胞后增强型绿色荧光蛋白的表达及细胞毒性,探讨适用于脂肪来源的成体干细胞的转基因方法。
AIM: To study the effects of egtazic acid (EA) and calcimycin (Cal) on the synthesis of DNA and collagen in cultured human lung fibroblasts (HLF). 目的:研究依他酸(EA)和卡西霉素(Cal)对人胚肺成纤维细胞脱氧核糖核酸和胶原合成的影响。
AIM:To investigate the pharmacokinetics of adefovir dipivoxil (ADV) with a single and multiple-dose oral administration in Chinese healthy volunteers. 目的:评估健康志愿者单次、多次口服阿德福韦酯片剂(ADV)的药动学。
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