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METHODS: Two recombinant pALTER plasmids containing two hmCD9 cDNAs were transfected into CHO cells respectively with pcDNA by lipofectamine.
中文意思:
方法: 分别构建两种含有hmCD9全长cDNA序列的重组pALTER质粒, 运用脂质体介导法, 与pcDNA 质粒共转染CHO细胞, 以G8筛选阳性克隆(编号为hmCD9- - CHO及hmCD9 -- CHO)。


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SD rats were immunosuppressed with injection of cortisone acetate .mg for weeks and the klatsch-preparations of lung were observed by high power with staining of giemsa and Gomori' methenamine silver nitrate stain. 给SD实验大鼠皮下注射醋酸可的松.mg/只,一周一次,从第三周开始陆续剖杀后制作实验大鼠肺组织印片。 经Giemsa染色及六亚甲基四胺银染色(Grocoti′s Methenamine-Silver nitrate Method,GMS染色),油镜观察。
METHODS The chromatographic column was Beckman ODS(.mm×cm,μm); 方法采用Beckman ODS柱(.mm×cm,μm),以乙腈-乙酸铵缓冲液为流动相,流速.0mL.
METHODS The concentration of Artificial bezoar was determined by measuring the UV absorbance at ±nm. 方法采用紫外分光光度法测定人工牛黄的含量,测定波长为 ±nm。
METHODS To summarizeand analyze the data of 0 cases with glossal valvi-form tracheotomy. 方法对 0例行舌瓣形气管切开术患者资料进行总结分析。
METHODS: The left ventricular myocardium specimens were obtained from 8 patients with dilated cardiomyopathy by BATISTA. The morphological changes was examined macropathologically and histopathologically. 方法 :利用BATISTA手术方法切取 8例扩张性心肌病患者左室心肌 ,进行大体、光镜及超微结构的形态学观察。
METHODS: Two recombinant pALTER plasmids containing two hmCD9 cDNAs were transfected into CHO cells respectively with pcDNA by lipofectamine. 方法: 分别构建两种含有hmCD9全长cDNA序列的重组pALTER质粒, 运用脂质体介导法, 与pcDNA 质粒共转染CHO细胞, 以G8筛选阳性克隆(编号为hmCD9- - CHO及hmCD9 -- CHO)。
METHODS: 8 cases of orbital facture associated with diplopia were examined by maxillofacial surgeon and oculist,by analyzing the CT and X-ray films of whom to make clear the reasons of diplopia. 方法:8例眶周骨折伴复视的患者,分别由口腔颌面外科、眼科医生检查,通过X线平片或CT片综合分析,明确复视的原因。
METHODS: ARSTRDB and simple inquest based on the World Health Organization Adverse Reaction Terms( WHO-ART) contained bynames was established. 研究方法以WHO药物不良反应术语集(World Health Organization Adverse Reaction Terms,WHO-ART)为基础建立包含别名的不良反应标准术语检索数据库,编写简单查询。
METHODS: Among the twenty eight patients (M9, F9; age 9 a± s a, course of disease mo± mo), there are tinea alba, kerion and tinea nigra. 方法 :选用 8例病人 ,男性 9例 ,女性 9例 ,年龄 9a±s a ,病程 mo± mo ,其中白癣 例 ,黑癣 例 ,脓癣 例。
METHODS: Biofilm model to quantify single-species (S.mutans ATCC7 or L. acidophilus ATCC) and dual-species (S.mutans ATCC7 and L.acidophilus ATCC) biofilm were formed in polystyrene 9-well microtiter plates for 8 h. 方法:变形链球菌(S.mutans,ATCC 7)和嗜酸乳杆菌(L.acidophilus,ATCC )在9孔板培养8h形成单菌种和双菌种生物膜。
METHODS: Frozen sections of the gamasid mites 0 days and more than 00 days after biting suckling mice inoculated with HFRSV were in situ hybridized with dig labelled HFRSV cDNA probes . 方法:用上海真厉螨与柏氏禽刺螨叮咬HFRSV接种乳小鼠,取叮咬后d0及d00以上的上海真厉螨与柏氏禽刺螨冰冻切片,用地高辛标记HFRSVcDNA核酸探针原位分子杂交检测其体内病毒RNA。
 
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