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Methods AML - ETO genomic fusion sequences were characterized by LD - PCR/LDI - PCR combined with sequence, then the patients specific primers were designed for the detection of AML - ETO fusion gene of the patients' Guthrie cards by PCr;
中文意思:
实验方法用LD-PCR或LDI-PCR联合序列分析确定t(8;)断裂区基因组DNA序列,根据所得序列设计病人特异性引物,用PCR方法检测患者Guthrie cards AML-ETO融合基因;


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Methods 8 patients above 0 years old with palpitation, stuffy chest, hard breath, lower ST-T, and low and flat T wave were analyzed by taking 0 mg of propranolol orally and observing ECG changes at 0, 0, 90 and 0 min. 方法分析8例0岁以上伴有心悸、胸闷、气短症状,静息心电图有ST段水平或下斜型压低,T波低平的患者,通过口服心得安药物0 mg,分别在 0、0、90、0 min描记心电图观察其变化。
Methods A HPLC method was used with DiamonsilTM C 8(0× . mn, μ m) column and a mobile phase of methanol- 0. % phospho ric acid solution (adjusted the pH to .0 with triethylamine)(0 ∶ 0). 方法采用HPLC法,流动相:甲醇-0.%磷酸(用三乙胺调pH至.0)(0∶0),色谱柱:DiamonsilTMC8(0×.mn,μm),流速:.0mL/min,柱温: ℃,波长:80nm,进样量:0μL。
Methods A PE-0 catheter with a silicon was used to block the middle cerebral artery(MCA) of rats for hours in cold saline infusion group,and locally infuse the MCA-supplied territory with . ml 0℃ saline for minutes before reperfusion. 方法应用内插有尼龙线的PE-0导管制作大鼠大脑中动脉(MCA)缺血 h模型,在再灌注前 min向MCA缺血区输注 . ml 0℃盐水,然后实施再灌注。
Methods A comparison study was made between 0 patients wearing monocles and 0 patients wearing progressive multi-focal spectacles about their degree of phoria taken with Von Graefe test at the start of wearing the spectacles and , months later. 方法用Von Graefe法测定0例单光眼镜配戴者及0例渐进多焦近视眼镜配戴者在配戴初,配戴后 、月隐斜量。
Methods A total of patients(mean age,7 years;age range, -9 years) with serum PSA> ng/ml or positive rectal examination results or abnormal B-ultrasonography findings underwent transperineal sextant biopsy and more cores of the nodules. 方法对 例PSA> ng/m l、直肠指检异常或B超发现异常回声的患者行经会阴的点系统加异常回声处活检。 年龄 ~9岁,平均7岁。
Methods AML - ETO genomic fusion sequences were characterized by LD - PCR/LDI - PCR combined with sequence, then the patients specific primers were designed for the detection of AML - ETO fusion gene of the patients' Guthrie cards by PCr; 实验方法用LD-PCR或LDI-PCR联合序列分析确定t(8;)断裂区基因组DNA序列,根据所得序列设计病人特异性引物,用PCR方法检测患者Guthrie cards AML-ETO融合基因;
Methods According to the 0 years' baseline data of AIDS of Xiamen City, the radices of the population at high risk for AIDS were estimated and the epidemic status of AIDS was evaluated by Workbook in Xiamen City. 方法依据厦门市0余年来积累的艾滋病监测及调查的基线数据,运用Work-book法,估计厦门市艾滋病高危人群基数,评估艾滋病疫情。
Methods Admission records of 9c hildren with ovarium cysts operated from Jua. 998to Dec.00,in cludi ngcases treated by routine open operation and8cases treated by laparoscop e were retrospectively analyzed,and was compared. 方法对比998年月至00年月 9例小儿卵巢囊肿手术治疗效果及预后,其中例行传统手术治疗,8例行腹腔镜手术。
Methods After ligating the splenic artery at pancreatic body or tail, the splenic injury was sutured preserving the arteriae gastricae breves and arteria gastroepiploica sinistra. 方法 剪开胃结肠韧带,在胰体尾后上方找到脾动脉后结扎,保留胃短动脉和胃网膜左动脉对脾脏的血供,并缝合脾裂口。
Methods After the relative surgical treatment of different cases of knee arthrosis pyogenic infection with kneescope,according to the principle of the dialectical method. The honeysuckle- amboyna liquid was used to lavage the location? 方法对例不同类型的膝关节感染病人在使用膝关节镜辅助下,进行相应外科处理后,按照辩证论治原则,使用银黄灌洗液进行局部灌洗。
Methods Amino acid sequence of LcrV on secondary structure, LcrV flexibility and hydrophilicity and antigenic index were analyzed using DNAStar Protean softeware and EMBOSS net platform to predict the B cell epitopes. 方法采用EMBOSS网络平台以及DNAStar Protean软件分析LcrV蛋白的二级结构、可塑性、亲水性和抗原性指数等参数,进行B细胞表位的预测和分析。
 
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