以下为句子列表：
英文: The tumor cell vaccine (TCV-m4-1BBL) was obtained by treating Hepa1-6-m4-1BBL with mitomycin (MMC). Cocultivation TCV with syngeneic murine spleen cells and the supernatants were harvested for detecting the cytokines (IL-2,TNF-α and GM-CSF).
中文: 方法以丝裂霉素C(MMC)处理高表达转m4-1BBL基因的小鼠Hepa1-6肝癌细胞,制成肿瘤细胞瘤苗(TCV),体外与同系小鼠脾淋巴细胞共同培养后,观察其对脾细胞产生细胞因子(IL-2、TNF-α和GM-CSF)的影响。
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