以下为句子列表：
英文: A gene fragment encoding human monocyte chemoattratctant protein-1 (MCP-1) was subcloned into pEX31A. MS2/MCP-1 fusion protein was expressed in E. coli and accounted for about 15% of total cell proteins.
中文: 将编码人单核细胞趋化蛋白－1（MCP－1）的基因亚克隆到大肠杆菌表达载体pEX31A中，在大肠杆菌中表达出MS2／MCP－1融合蛋0白，该表达产物约占菌体总蛋白的15％左右，Westernblot检测表明，表达产物可与MCP－1抗体特异反应。
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英文: Conclusions TNF-α,MCP-1 are cytokines which were activated in sepsis early,and their release is related with NF-κB activity.
中文: 结论TNF-α、MCP-1是脓毒症早期激活的细胞因子,它们的释放与NF-κB的活性密切相关;
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